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Literature summary extracted from

  • Wu, S.M.; Cheung, W.F.; Frazier, D.; Stafford, D.W.
    Cloning and expression of the cDNA for human gamma-glutamyl carboxylase (1991), Science, 13, 1634-1636.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.1.90 ammonium sulfate
-
 Homo sapiens
4.1.1.90 proFIX 19
-
 Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.90 expression of the cloned cDNA results in an increase in carboxylase activity in microsomes of transfected cells compared to mock-transfected cells Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.1.1.90 membrane 758 residue integral membrane protein Homo sapiens 16020
-
4.1.1.90 microsome microsomal carboxylase activity is compared from cells transfected with pCMV5 Homo sapiens
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.90 87540
-
 calculated from sequence Homo sapiens
4.1.1.90 94000
-
 SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.90 additional information Homo sapiens enzyme accomplishes the post-translational modification required for the activity of all of the vitamin K-dependent proteins ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.90 Homo sapiens P38435
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.1.90 HEK-293 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.90 FLEEL + CO2 + O2 + ammonium sulfate carboxylase activity is measured by 14CO2 incorporation into the synthetic peptide substrate FLEEL Homo sapiens ? + vitamin K epoxide + H2O
-
 ?
4.1.1.90 FLEEL + CO2 + O2 + proFIX 19 carboxylase activity is measured by 14CO2 incorporation into the synthetic peptide substrate FLEEL Homo sapiens ? + vitamin K epoxide + H2O
-
 ?
4.1.1.90 FLEEL + CO2 + O2 + vitamin KH2 carboxylase activity is measured by 14CO2 incorporation into the synthetic peptide substrate FLEEL Homo sapiens ? + vitamin K epoxide + H2O
-
 ?
4.1.1.90 L-glutamate + CO2 + O2 + vitamin K hydroquinone reaction is essential for the activity of all of the vitamin K-dependent proteins Homo sapiens gamma-carboxy-L-glutamate + vitamin K epoxide + H2O
-
 ?
4.1.1.90 additional information enzyme accomplishes the post-translational modification required for the activity of all of the vitamin K-dependent proteins Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.90 ? x * 94000, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
4.1.1.90 gamma-glutamyl carboxylase
-
 Homo sapiens
4.1.1.90 glutamate carboxylase
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.90 vitamin K carboxylase and soybean seed lipoxygenase share 19.3% identity over a span of 198 amino acids, from residues 468 to 666 of carboxylase. This is interesting because the carboxylase acts as an oxygenase on the cofactor vitamin K-hydroquinone, and the similarity occurs in that region of the carboxylase likely to have enzymatic function Homo sapiens