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Literature summary extracted from

  • Mark, P.; Baumann, M.J.; Ekloef, J.M.; Gullfot, F.; Michel, G.; Kallas, A.M.; Teeri, T.T.; Brumer, H.; Czjzek, M.
    Analysis of nasturtium TmNXG1 complexes by crystallography and molecular dynamics provides detailed insight into substrate recognition by family GH16 xyloglucan endo-transglycosylases and endo-hydrolases (2008), Proteins, 75, 820-836.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.207 expressed in Pichia pastoris strain GS115 Tropaeolum majus
3.2.1.151 heterologous expression in Pichia pastoris strain GS115 Tropaeolum majus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.1.207 active-site loop deletion variant NXG1-DELTAYNIIG in free and product-complexed forms, using 0.2 M sodium formate, 0.1 M Bis Tris propane buffer at pH 6.5, and 20% (w/v) PEG 3350 Tropaeolum majus
3.2.1.151 loop mutant TmNXG1-DELTAYNIIG with an oligosaccharide product bound in the negative active-site subsites, X-ray diffraction structure determination and analysis at 2.0 A resolution, molecular replacement, molecular dynamics simulations and structure modelling of wild-type and mutant enzyme, detailed overview Tropaeolum majus
3.2.1.151 TmNXG1-deltaYNIIG with bound substrate xylogluco-oligosaccharide Tropaeolum majus

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.151 additional information TmNXG1-deltaYNIIG: active site loop deletion mutant, three loops: Asn84-Asp93 (glycosyl donor subsite), Glu117-Gly126, and Trp190-Tyr197 (critical to acceptor substrate binding, inter alia influencing transglycosylation/hydrolysis ratio) Tropaeolum majus
3.2.1.151 additional information construction of a loop mutant TmNXG1-DELTAYNIIG Tropaeolum majus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.151 additional information
-
 additional information modelling of Michaelis complexes of wild-type and mutant enzyme, overview Tropaeolum majus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.151 additional information Tropaeolum majus nasturtium xyloglucanase 1, predominant endo-hydrolase of Tropaeolum majus that can also perform xyloglucan endo-transglycosylation at elevated substrate concentrations ?
-
 ?
3.2.1.151 xyloglucan + H2O Tropaeolum majus
-
 xyloglucan oligosaccharides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.207 Tropaeolum majus
-
-
-
3.2.1.151 Tropaeolum majus
-
-
-
3.2.1.151 Tropaeolum majus
-
 nasturtium
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.151 concentration with Centricon devices, cutoff 10 kDa Tropaeolum majus

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.207 donor xyloglucan + acceptor xyloglucan
-
 Tropaeolum majus ?
-
 ?
3.2.1.151 additional information nasturtium xyloglucanase 1, predominant endo-hydrolase of Tropaeolum majus that can also perform xyloglucan endo-transglycosylation at elevated substrate concentrations Tropaeolum majus ?
-
 ?
3.2.1.151 octodecasaccharide XLLG-XLLG + H2O xylogluco-nonasaccharide Tropaeolum majus ?
-
 ?
3.2.1.151 xyloglucan + H2O
-
 Tropaeolum majus xyloglucan oligosaccharides
-
 ?
3.2.1.151 xyloglucan + H2O tamarind seed xyloglucan Tropaeolum majus xyloglucan oligosaccharides
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.151 More importance of various loops lining the active site. Subtle differences leading to a tighter hydrogen bonding pattern on the negative, glycosyl donor, binding subsites, together with loop flexibility on the positive, glycosyl acceptor, binding subsites appear to favor hydrolysis over transglycosylation in GH16 xyloglucan-active enzymes Tropaeolum majus

Synonyms

EC Number Synonyms Comment Organism
2.4.1.207 endo-xyloglucanase
-
 Tropaeolum majus
2.4.1.207 NXG1
-
 Tropaeolum majus
2.4.1.207 XET
-
 Tropaeolum majus
2.4.1.207 xyloglucan endo-transglycosylase
-
 Tropaeolum majus
2.4.1.207 xyloglucanase 1
-
 Tropaeolum majus
3.2.1.151 family GH16 xyloglucan endo-transglycosylase
-
 Tropaeolum majus
3.2.1.151 More the enzyme belongs to the glycoside hydrolase family 16, GH16 Tropaeolum majus
3.2.1.151 TmNXG1
-
 Tropaeolum majus
3.2.1.151 xyloglucan endotransglycosylase/hydrolase
-
 Tropaeolum majus
3.2.1.151 xyloglucanase 1
-
 Tropaeolum majus