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Literature summary extracted from

  • Martinez-Martinez, I.; Navarro-Fernandez, J.; Garcia-Carmona, F.; Takami, H.; Sanchez-Ferrer, A.
    Characterization and structural modeling of a novel thermostable glycine oxidase from Geobacillus kaustophilus HTA426 (2008), Proteins Struct. Funct. Bioinform., 70, 1429-1441.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.3.19 expression in Escherichia coli Geobacillus kaustophilus HTA426

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.19 0.22
-
 N-ethylglycine pH 8.5, 37°C Geobacillus kaustophilus HTA426
1.4.3.19 0.25
-
 glycine pH 8.5, 37°C Geobacillus kaustophilus HTA426
1.4.3.19 0.45
-
 sarcosine pH 8.5, 37°C Geobacillus kaustophilus HTA426
1.4.3.19 0.52
-
 glycine-ethyl-ester pH 8.5, 37°C Geobacillus kaustophilus HTA426
1.4.3.19 9.3
-
 D-proline pH 8.5, 37°C Geobacillus kaustophilus HTA426
1.4.3.19 20.6
-
 D-alanine pH 8.5, 37°C Geobacillus kaustophilus HTA426
1.4.3.19 221.8
-
 D-pipecolate pH 8.5, 37°C Geobacillus kaustophilus HTA426

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.19 43000
-
 4 * 43000, SDS-PAGE Geobacillus kaustophilus HTA426
1.4.3.19 160000
-
 gel filtration Geobacillus kaustophilus HTA426

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.19 Geobacillus kaustophilus HTA426
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.3.19 11.85
-
 pH 8.5, 37°C Geobacillus kaustophilus HTA426

Storage Stability

EC Number Storage Stability Organism
1.4.3.19 -80°C, 75 mM sodium diphosphate, pH 8.5, 10% glycerol, stable for months Geobacillus kaustophilus HTA426

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.19 cephalosporin C + H2O + O2
-
 Geobacillus kaustophilus HTA426 ?
-
 ?
1.4.3.19 D-Ala + H2O + O2
-
 Geobacillus kaustophilus HTA426 ? + NH3 + H2O2
-
 ?
1.4.3.19 D-arginine + H2O + O2
-
 Geobacillus kaustophilus HTA426 ?
-
 ?
1.4.3.19 D-aspartate + H2O + O2
-
 Geobacillus kaustophilus HTA426 ?
-
 ?
1.4.3.19 D-methionine + H2O + O2
-
 Geobacillus kaustophilus HTA426 4-(methylsulfanyl)-2-oxobutanoic acid + NH3 + H2O2
-
 ?
1.4.3.19 D-norvaline + H2O + O2
-
 Geobacillus kaustophilus HTA426 2-oxopentanoic acid + NH3 + H2O2
-
 ?
1.4.3.19 D-phenylalanine + H2O + O2
-
 Geobacillus kaustophilus HTA426 phenylpyruvate + NH3 + H2O2
-
 ?
1.4.3.19 D-pipecolate + H2O + O2
-
 Geobacillus kaustophilus HTA426 ?
-
 ?
1.4.3.19 D-Pro + H2O + O2
-
 Geobacillus kaustophilus HTA426 ? + NH3 + H2O2
-
 ?
1.4.3.19 D-valine + H2O + O2
-
 Geobacillus kaustophilus HTA426 3-methyl-2-oxobutanoic acid + NH3 + H2O2
-
 ?
1.4.3.19 glycine + H2O + O2
-
 Geobacillus kaustophilus HTA426 glyoxylate + NH3 + H2O2
-
 ?
1.4.3.19 glycine-ethyl-ester + H2O + O2
-
 Geobacillus kaustophilus HTA426 ? + H2O2
-
 ?
1.4.3.19 additional information substrate preference for amines of small size, like glycine, sarcosine, N-ethyl-glycine, and glycine-ethylester Geobacillus kaustophilus HTA426 ?
-
 ?
1.4.3.19 N-ethylglycine + H2O + O2
-
 Geobacillus kaustophilus HTA426 glyoxylate + ethylamine + H2O2
-
 ?
1.4.3.19 sarcosine + H2O + O2
-
 Geobacillus kaustophilus HTA426 glyoxylate + methylamine + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.3.19 tetramer 4 * 43000, SDS-PAGE Geobacillus kaustophilus HTA426

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.3.19 74
-
-
 Geobacillus kaustophilus HTA426

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.4.3.19 60
-
 activation during the first 15 min, with slow decrease thereafter Geobacillus kaustophilus HTA426

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.3.19 80
-
 half-life 0.6 h Geobacillus kaustophilus HTA426

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.3.19 8.5
-
-
 Geobacillus kaustophilus HTA426