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Literature summary extracted from

  • Conrath, K.; Pereira, A.S.; Martins, C.E.; Timoteo, C.G.; Tavares, P.; Spinelli, S.; Kinne, J.; Flaudrops, C.; Cambillau, C.; Muyldermans, S.; Moura, I.; Moura, J.J.; Tegoni, M.; Desmyter, A.
    Camelid nanobodies raised against an integral membrane enzyme, nitric oxide reductase (2009), Protein Sci., 18, 619-628.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.7.2.5 in complex with variable heavy chain domain of camel heavy chain antibody, sitting drop vapor diffusion method, using 100 mM potassium phosphate pH 7.0, 0.01% (v/v) phenyl ethanol, 0.005% (v/v) n-dodecyl-beta-D-maltopyranoside Marinobacter nauticus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.7.2.5 membrane integral membrane enzyme Marinobacter nauticus 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.2.5 17600
-
 subunit NorC, gel filtration, not detectable by mass spectrometry Marinobacter nauticus
1.7.2.5 38000
-
 subunit NorB, gel filtration, not detectable by mass spectrometry Marinobacter nauticus
1.7.2.5 54400
-
 subunit NorB, calculated from amino acid sequence Marinobacter nauticus
1.7.2.5 71800
-
 NOR enzyme complex, calculated from amino acid sequence Marinobacter nauticus

Organism

EC Number Organism UniProt Comment Textmining
1.7.2.5 Marinobacter nauticus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.2.5 DEAE column chromatography and hydroxyapatite column chromatography Marinobacter nauticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.2.5 nitric oxide + ferrocytochrome c552
-
 Marinobacter nauticus nitrous oxide + ferricytochrome c552 + H2O
-
 ?
1.7.2.5 nitric oxide + reduced acceptor
-
 Marinobacter nauticus nitrous oxide + oxidized acceptor + H2O
-
 ?
1.7.2.5 nitric oxide + reduced ascorbate
-
 Marinobacter nauticus nitrous oxide + oxidized ascorbate + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.7.2.5 nitric oxide reductase
-
 Marinobacter nauticus
1.7.2.5 NOR
-
 Marinobacter nauticus
1.7.2.5 NorB subunit Marinobacter nauticus
1.7.2.5 NorC subunit Marinobacter nauticus

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.2.5 heme
-
 Marinobacter nauticus