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Literature summary extracted from

  • Pakhomova, S.; Gao, B.; Boeglin, W.E.; Brash, A.R.; Newcomer, M.E.
    The structure and peroxidase activity of a 33kD catalase-related protein from Mycobacterium avium ssp. paratuberculosis (2009), Protein Sci., 18, 2559-2568.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.11.1.7 hanging drop vapour diffusion method, using 1.5 M 1,6-hexanediol, 100 mM sodium citrate pH 6.9, 13-15% PEG 20000, and 2.5-6.5% glycerol Mycobacterium avium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.7 3
-
 cumene hydroperoxide in 50 mM potassium phosphate buffer, pH 7.0 Mycobacterium avium
1.11.1.7 22
-
 tert-butyl hydroperoxide in 50 mM potassium phosphate buffer, pH 7.0 Mycobacterium avium
1.11.1.7 30
-
 H2O2 in 50 mM potassium phosphate buffer, pH 7.0 Mycobacterium avium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.7 33000
-
 subunit, SDS-PAGE Mycobacterium avium

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.7 Mycobacterium avium
-
 subspecies Mycobacterium avium paratuberculosis
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.7 Ni-NTA column chromatography, TALON Co2+ affinity column chromatography, and Superdex 200 gel filtration Mycobacterium avium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.7 2,2'-azino-bis-(3-ethylbenzthiazole-6-sulfonic acid) + (13S)-hydroperoxylinoleic acid
-
 Mycobacterium avium ?
-
 ?
1.11.1.7 2,2'-azino-bis-(3-ethylbenzthiazole-6-sulfonic acid) + (5S)-hydroperoxyarachidonic acid
-
 Mycobacterium avium ?
-
 ?
1.11.1.7 2,2'-azino-bis-(3-ethylbenzthiazole-6-sulfonic acid) + (8R)-hydroperoxyarachidonic acid
-
 Mycobacterium avium ?
-
 ?
1.11.1.7 2,2'-azino-bis-(3-ethylbenzthiazole-6-sulfonic acid) + (9S)-hydroperoxylinoleic acid
-
 Mycobacterium avium ?
-
 ?
1.11.1.7 2,2'-azino-bis-(3-ethylbenzthiazole-6-sulfonic acid) + cumene hydroperoxide
-
 Mycobacterium avium ?
-
 ?
1.11.1.7 2,2'-azino-bis-(3-ethylbenzthiazole-6-sulfonic acid) + H2O2
-
 Mycobacterium avium ?
-
 ?
1.11.1.7 2,2'-azino-bis-(3-ethylbenzthiazole-6-sulfonic acid) + tert-butyl hydroperoxide
-
 Mycobacterium avium ?
-
 ?
1.11.1.7 additional information MAP-2744c exhibits strong peroxidase activity using fatty acid hydroperoxides Mycobacterium avium ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.11.1.7 MAP-2744c
-
 Mycobacterium avium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.7 13
-
 H2O2 in 50 mM potassium phosphate buffer, pH 7.0 Mycobacterium avium
1.11.1.7 320
-
 tert-butyl hydroperoxide in 50 mM potassium phosphate buffer, pH 7.0 Mycobacterium avium
1.11.1.7 330
-
 cumene hydroperoxide in 50 mM potassium phosphate buffer, pH 7.0 Mycobacterium avium

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.7 heme
-
 Mycobacterium avium