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Literature summary extracted from

  • Reddy, M.C.; Kuppan, G.; Shetty, N.D.; Owen, J.L.; Ioerger, T.R.; Sacchettini, J.C.
    Crystal structures of Mycobacterium tuberculosis S-adenosyl-L-homocysteine hydrolase in ternary complex with substrate and inhibitors (2008), Protein Sci., 17, 2134-2144.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.13.2.1 expressed in Escherichia coli as a His-tagged fusion protein Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.13.2.1 crystal structure of SAHH in complex with adenosine (ADO) and nicotinamide adenine dinucleotide and with three inhibitors is shown: 3'-keto aristeromycin, 2-fluoroadenosine, and 3-deazaadenosine. The 3'-keto aristeromycin complex is the first reported structure of SAHH complexed with this inhibitor, and confirms the oxidation of the 3'-hydroxyl to a planar keto group, consistent with its prediction as a mechanism-based inhibitor. The 2.0 A resolution structure of the complex of SAHH cocrystallized with S-adenosylhomocysteine is reported. Binding of homocysteine forces a rotation of His363 around the backbone to flip out of contact with the 5’ hydroxyl of the adenosine and opens access to a nearby channel that leads to the surface. This complex suggests that His363 acts as a switch that opens up to permit binding of substrate, then closes down after release of the cleaved homocysteine Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.13.2.1 2-fluoroadenosine
-
 Mycobacterium tuberculosis
3.13.2.1 3'-keto aristeromycin
-
 Mycobacterium tuberculosis
3.13.2.1 3-deazaadenosine
-
 Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
3.13.2.1 Mycobacterium tuberculosis P9WGV3
-
-
3.13.2.1 Mycobacterium tuberculosis H37Rv P9WGV3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.13.2.1 using Ni-NTA chromatography Mycobacterium tuberculosis

Subunits

EC Number Subunits Comment Organism
3.13.2.1 homotetramer crystal structure Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
3.13.2.1 S-adenosyl-L-homocysteine hydrolase
-
 Mycobacterium tuberculosis
3.13.2.1 SAHH
-
 Mycobacterium tuberculosis