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Literature summary extracted from

  • Vetting, M.W.; Roderick, S.L.; Yu, M.; Blanchard, J.S.
    Crystal structure of mycothiol synthase (Rv0819) from Mycobacterium tuberculosis shows structural homology to the GNAT family of N-acetyltransferases (2003), Protein Sci., 12, 1954-1959.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.189 the Rv0819 gene encoding MshD (residues 1–315) is amplified from Mycobacterium tuberculosis genomic DNA using PCR and cloned into the pET28a(+) plasmid. This vector construction adds six histidine residues and a thrombin cleavage sequence to the N-terminus to facilitate protein purification. Expression in Escherichia coli stain BL21(DE3)pLysS Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.189 vapor diffusion under oil method Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.189 desacetylmycothiol + acetyl-CoA Mycobacterium tuberculosis
-
 mycothiol + coenzyme-A
-
 ?
2.3.1.189 desacetylmycothiol + acetyl-CoA Mycobacterium tuberculosis H37Rv
-
 mycothiol + coenzyme-A
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.189 Mycobacterium tuberculosis P9WJM7
-
-
2.3.1.189 Mycobacterium tuberculosis H37Rv P9WJM7
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.189 desacetylmycothiol + acetyl-CoA
-
 Mycobacterium tuberculosis mycothiol + coenzyme-A
-
 ?
2.3.1.189 desacetylmycothiol + acetyl-CoA
-
 Mycobacterium tuberculosis H37Rv mycothiol + coenzyme-A
-
 ?