EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.1.21.1 | additional information | His44 may play a critical role in substrate DNA binding in the putative secondary active site, and introduction of sulfhydryl groups at Thr14 and Ser43 may facilitate Mn2+-coordination and further contribute to the catalytic activity of DNase I | Bos taurus |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.21.1 | into vector pET15b and expressed in Escherichia coli strain BL21(DE3)pLysE | Bos taurus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.1.21.1 | H44A | DNase I activity is similar to that of the wild-type | Bos taurus |
3.1.21.1 | H44D | inactive, can only cleave the Mn2+-DNA substrate in a single nicking mode | Bos taurus |
3.1.21.1 | S43A | in the presence of Mn2+, as the wild-type, is able to hydrolyze the Mn2+-substrate forming-linear duplex DNA in addition to the relaxed open-circular DNA, indicating double scission | Bos taurus |
3.1.21.1 | S43A/H44D | inactive, can only cleave the Mn2+-DNA substrate in a single nicking mode | Bos taurus |
3.1.21.1 | S43C | in the presence of Mn2+, as the wild-type, is able to hydrolyze the Mn2+-substrate forming-linear duplex DNA in addition to the relaxed open-circular DNA, indicating double scission | Bos taurus |
3.1.21.1 | T14A | in the presence of Mn2+, as the wild-type, is able to hydrolyze the Mn2+-substrate forming-linear duplex DNA in addition to the relaxed open-circular DNA, indicating double scission | Bos taurus |
3.1.21.1 | T14A/H44D | inactive, can only cleave the Mn2+-DNA substrate in a single nicking mode | Bos taurus |
3.1.21.1 | T14A/S43A | in the presence of Mn2+, though being less active than the wild-type, can still cleave the plasmid DNA in the double scission mode | Bos taurus |
3.1.21.1 | T14A/S43A/H44D | inactive | Bos taurus |
3.1.21.1 | T14A/S43C | DNase I activity is similar to that of the wild-type | Bos taurus |
3.1.21.1 | T14C | in the presence of Mn2+, as the wild-type, is able to hydrolyze the Mn2+-substrate forming-linear duplex DNA in addition to the relaxed open-circular DNA, indicating double scission | Bos taurus |
3.1.21.1 | T14C/H44D | significant increase in DNase I activity | Bos taurus |
3.1.21.1 | T14C/S43A | DNase I activity is similar to that of the wild-type | Bos taurus |
3.1.21.1 | T14C/S43A/H44D | significant increase in DNase I activity | Bos taurus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.21.1 | Ca2+ | - |
Bos taurus | |
3.1.21.1 | Mg2+ | in the presence of Mg2+ only, the wild-type cleaves the Mg2+-DNA substrate in a single nicking mode with the formation of only the relaxed open-circular DNA | Bos taurus | |
3.1.21.1 | Mn2+ | in the presence of Mn2+, the wild-type is able to hydrolyze the Mn2+-substrate forming-linear duplex DNA in addition to the relaxed open-circular DNA, indicating double scission | Bos taurus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.21.1 | Bos taurus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.21.1 | to homogeneity | Bos taurus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.21.1 | pancreas | - |
Bos taurus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.21.1 | calf thymus DNA + H2O | - |
Bos taurus | 5'-phosphooligonucleotides + ? | - |
? | |
3.1.21.1 | plasmid DNA + H2O | - |
Bos taurus | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.21.1 | DNase I | - |
Bos taurus |