Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Lin, L.L.; Chen, Y.P.; Yang, J.C.; Hua, Y.W.; Wang, W.C.; Kuo, L.Y.
    Significance of the conserved Tyr352 and Asp380 residues in the catalytic activity of Bacillus stearothermophilus aminopeptidase II as evaluated by site-directed mutagenesis (2008), Protein J., 27, 215-222.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.10 expressed in Escherichia coli M15 cells Geobacillus stearothermophilus

Protein Variants

EC Number Protein Variants Comment Organism
3.4.11.10 D380E the mutation causes more than 2fold decrease in kcat as compared to the wild type enzyme Geobacillus stearothermophilus
3.4.11.10 D380N the mutation causes more than 2fold decrease in kcat as compared to the wild type enzyme Geobacillus stearothermophilus
3.4.11.10 D380V the mutation causes over 5fold decrease in kcat as compared to the wild type enzyme Geobacillus stearothermophilus
3.4.11.10 Y352D the mutation results in about 3.5fold decrease in specific activity Geobacillus stearothermophilus
3.4.11.10 Y352L the mutation results in about 3.5fold decrease in specific activity Geobacillus stearothermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.10 0.95
-
 L-Leu-4-nitroanilide wild type enzyme, in 25 mM Tris-HCl buffer, pH 8.0, at 60°C Geobacillus stearothermophilus
3.4.11.10 2.12
-
 L-Leu-4-nitroanilide mutant enzyme Y352D, in 25 mM Tris-HCl buffer, pH 8.0, at 60°C Geobacillus stearothermophilus
3.4.11.10 2.83
-
 L-Leu-4-nitroanilide mutant enzyme Y352L, in 25 mM Tris-HCl buffer, pH 8.0, at 60°C Geobacillus stearothermophilus
3.4.11.10 3.05
-
 L-Leu-4-nitroanilide mutant enzyme D380N, in 25 mM Tris-HCl buffer, pH 8.0, at 60°C Geobacillus stearothermophilus
3.4.11.10 3.39
-
 L-Leu-4-nitroanilide mutant enzyme D380E, in 25 mM Tris-HCl buffer, pH 8.0, at 60°C Geobacillus stearothermophilus
3.4.11.10 3.41
-
 L-Leu-4-nitroanilide mutant enzyme D380V, in 25 mM Tris-HCl buffer, pH 8.0, at 60°C Geobacillus stearothermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.10 45000
-
 SDS-PAGE Geobacillus stearothermophilus

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.10 Geobacillus stearothermophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.10 Ni2+-NTA resin chromatography Geobacillus stearothermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.11.10 13.8
-
 mutant enzyme D380E, at 60°C, using L-Leu-4-nitroanilide as substrate Geobacillus stearothermophilus
3.4.11.10 15.1
-
 mutant enzyme D380V, at 60°C, using L-Leu-4-nitroanilide as substrate Geobacillus stearothermophilus
3.4.11.10 34.5
-
 mutant enzyme Y532L, at 60°C, using L-Leu-4-nitroanilide as substrate Geobacillus stearothermophilus
3.4.11.10 55.3
-
 mutant enzyme D380N, at 60°C, using L-Leu-4-nitroanilide as substrate Geobacillus stearothermophilus
3.4.11.10 104.9
-
 mutant enzyme Y532D, at 60°C, using L-Leu-4-nitroanilide as substrate Geobacillus stearothermophilus
3.4.11.10 572.5
-
 wild type enzyme, at 60°C, using L-Leu-4-nitroanilide as substrate Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.10 L-Leu-4-nitroanilide + H2O
-
 Geobacillus stearothermophilus L-Leu + 4-nitroaniline
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.11.10 aminopeptidase II
-
 Geobacillus stearothermophilus
3.4.11.10 AP-II
-
 Geobacillus stearothermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.11.10 0.035
-
 L-Leu-4-nitroanilide mutant enzyme D380E, in 25 mM Tris-HCl buffer, pH 8.0, at 60°C Geobacillus stearothermophilus
3.4.11.10 0.048
-
 L-Leu-4-nitroanilide mutant enzyme D380V, in 25 mM Tris-HCl buffer, pH 8.0, at 60°C Geobacillus stearothermophilus
3.4.11.10 0.113
-
 L-Leu-4-nitroanilide mutant enzyme Y352L, in 25 mM Tris-HCl buffer, pH 8.0, at 60°C Geobacillus stearothermophilus
3.4.11.10 0.135
-
 L-Leu-4-nitroanilide mutant enzyme D380N, in 25 mM Tris-HCl buffer, pH 8.0, at 60°C Geobacillus stearothermophilus
3.4.11.10 0.153
-
 L-Leu-4-nitroanilide mutant enzyme Y352D, in 25 mM Tris-HCl buffer, pH 8.0, at 60°C Geobacillus stearothermophilus
3.4.11.10 0.303
-
 L-Leu-4-nitroanilide wild type enzyme, in 25 mM Tris-HCl buffer, pH 8.0, at 60°C Geobacillus stearothermophilus