Any feedback?
Literature summary extracted from
- Improved expression and purification of human multidrug resistance protein MDR1 from baculovirus-infected insect cells (2009), Protein Expr. Purif., 66, 7-14.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 7.6.2.2 | MDR1, large-scale expression of His10-tagged human MDR1 using a baculovirus/insect expressSF+ cell system | Homo sapiens |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 7.6.2.2 | membrane | - |
Homo sapiens | 16020 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.6.2.2 | Mg2+ | required | Homo sapiens |  |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.6.2.2 | ATP + H2O + xenobiotic/in | Homo sapiens | multidrug resistance protein MDR1, i.e. P-glycoprotein or ABCB1, is an ATP-dependent efflux pump for various cytotoxic agents, and is implicated in the resistance of human tumors to chemotherapeutic drugs | ADP + phosphate + xenobiotic/out | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 7.6.2.2 | Homo sapiens | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 7.6.2.2 | recombinant His10-tagged MDR1 249fold by metal chelate affinity chromatography and gel filtration, method optimization and evaluation | Homo sapiens |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 7.6.2.2 | 1.7 | - |
purified recombinant wild-type MDR1, ATPase activity with substrate verapamil | Homo sapiens |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 7.6.2.2 | most suitable component for maintaining hMDR1 activity is PIPES, rather than citric acid, imidazole, MES, or MOPS. Optimal pH for stabilizing the recombinant enzyme protein during purification is pH 6.5. Addition of CHS preserves 90% of the activity at 25°C after 1 day, whereas little or no activity remains without CHS. The specific ATPase activity is completely diminished after 2 h at 37°C, but 75% of the ATPase activity remains after 24 h at 10°C | Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.6.2.2 | ATP + H2O + verapamil/in | - |
Homo sapiens | ADP + phosphate + verapamil/out | - |
? | |
| 7.6.2.2 | ATP + H2O + xenobiotic/in | - |
Homo sapiens | ADP + phosphate + xenobiotic/out | - |
? | |
| 7.6.2.2 | ATP + H2O + xenobiotic/in | multidrug resistance protein MDR1, i.e. P-glycoprotein or ABCB1, is an ATP-dependent efflux pump for various cytotoxic agents, and is implicated in the resistance of human tumors to chemotherapeutic drugs | Homo sapiens | ADP + phosphate + xenobiotic/out | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 7.6.2.2 | More | three-dimensional structural analysis, overview | Homo sapiens |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 7.6.2.2 | ABCB1 | - |
Homo sapiens |
| 7.6.2.2 | MDR1 | - |
Homo sapiens |
| 7.6.2.2 | multidrug resistance protein | - |
Homo sapiens |
| 7.6.2.2 | P-glycoprotein | - |
Homo sapiens |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 7.6.2.2 | 37 | - |
assay at | Homo sapiens |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 7.6.2.2 | 10 | 37 | the specific ATPase activity is completely diminished after 2 h at 37°C, but 75% of the ATPase activity remains after 24 h at 10°C | Homo sapiens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 7.6.2.2 | 7.4 | - |
assay at | Homo sapiens |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 7.6.2.2 | 6.5 | - |
optimal pH for stabilizing the recombinant enzyme protein during purification is pH 6.5. hMDR1 remains monodispersed at a pH range of pH 5.5-pH 7.5. In contrast, at a lower pH such as pH 4.5, the protein is precipitated | Homo sapiens |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.6.2.2 | ATP | - |
Homo sapiens | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
