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Literature summary extracted from

  • Quintas-Granados, L.I.; Orozco, E.; Brieba, L.G.; Arroyo, R.; Ortega-Lopez, J.
    Purification, refolding and autoactivation of the recombinant cysteine proteinase EhCP112 from Entamoeba histolytica (2009), Protein Expr. Purif., 63, 26-32.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.35 recombinant expression of the His-tagged enzyme in Escherichia coli inclusion bodies Entamoeba histolytica

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.35 chymostatin
-
 Entamoeba histolytica
3.4.22.35 E-64
-
 Entamoeba histolytica
3.4.22.35 Nalpha-4-tosyl-L-lysine chloromethylketone
-
 Entamoeba histolytica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.35 35500
-
 x * 52000, recombinant and refolded enzyme, SDS-PAGE, x * 35500, recombinant, refolded, and processed enzyme, SDS-PAGE Entamoeba histolytica
3.4.22.35 52000
-
 x * 52000, recombinant and refolded enzyme, SDS-PAGE, x * 35500, recombinant, refolded, and processed enzyme, SDS-PAGE Entamoeba histolytica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.35 additional information Entamoeba histolytica the cysteine proteinase EhCP112 and the adhesin EhADH112 assemble to form the EhCPADH complex involved in Entamoeba histolytica virulence ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.35 Entamoeba histolytica
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.22.35 proteolytic modification the recombinant enzyme needs to be refolded and processed from the inactive precursor, ppEhCP112, to a 35.5 kDa mature and active enzyme, EhCP112. The thiol specific inhibitor E-64, but not serine or aspartic proteinase inhibitors arrests this activation process Entamoeba histolytica

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.35 recombinant refolded His-tagged enzyme by nickel affinity chromatography Entamoeba histolytica

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.4.22.35 recombinant His-tagged enzyme, solvation in 20 mM sodium phosphate, pH 7.4, 0.5 M NaCl, 10 mM imidazole, and 8 M urea, refolding and processing of the inactive precursor, ppEhCP112, to a 35.5 kDa mature and active enzyme, EhCP112 Entamoeba histolytica

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.22.35 1450
-
 refolded, recombinant, activated enzyme Entamoeba histolytica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.35 azocasein + H2O
-
 Entamoeba histolytica ?
-
 ?
3.4.22.35 additional information the cysteine proteinase EhCP112 and the adhesin EhADH112 assemble to form the EhCPADH complex involved in Entamoeba histolytica virulence Entamoeba histolytica ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.35 ? x * 52000, recombinant and refolded enzyme, SDS-PAGE, x * 35500, recombinant, refolded, and processed enzyme, SDS-PAGE Entamoeba histolytica

Synonyms

EC Number Synonyms Comment Organism
3.4.22.35 EhCP112
-
 Entamoeba histolytica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.22.35 37
-
 assay at, substrate azocasein Entamoeba histolytica

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.22.35 5 60
-
 Entamoeba histolytica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.35 7
-
 assay at, substrate azocasein Entamoeba histolytica

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.22.35 3 8
-
 Entamoeba histolytica