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Literature summary extracted from

  • Bashiri, G.; Squire, C.J.; Baker, E.N.; Moreland, N.J.
    Expression, purification and crystallization of native and selenomethionine labeled Mycobacterium tuberculosis FGD1 (Rv0407) using a Mycobacterium smegmatis expression system (2007), Protein Expr. Purif., 54, 38-44.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.98.2 initial attempts to produce recombinant FGD1 using Escherichia coli as a host is unsuccessful, but when the alternative host Mycobacterium smegmatis is used, soluble protein yields of 7 mg/l of culture are achieved Mycobacterium tuberculosis
1.1.98.2 recombinant expression of His-tagged wild-type and selenomethionine-labeled FGD1 in Mycobacterium smegmatis strain mc24517 Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.98.2 recombinant wild-type and selenomethionine-labeled FGD1, sitting drop vapour diffusion, mixing of protein solution in a 1:1 ratio with precipitant solution containing 1.6 M tri-sodium citrate, pH 6.5, microseeding, X-ray diffraction structure determination and analysis at 2.1 A resolution, multiwavelength anomalous diffraction Mycobacterium tuberculosis
1.1.98.2 sitting drop vapour diffusion method, native and selenomethionine-labeled FGD1 are successfully crystallized by vapor diffusion, with the crystals diffracting to 2.1 A resolution Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.98.2 D-glucose 6-phosphate + oxidized coenzyme F420 Mycobacterium tuberculosis
-
 D-glucono-1,5-lactone 6-phosphate + reduced coenzyme F420
-
 ?
1.1.98.2 D-glucose 6-phosphate + oxidized coenzyme F420 Mycobacterium tuberculosis H37Rv
-
 D-glucono-1,5-lactone 6-phosphate + reduced coenzyme F420
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.98.2 Mycobacterium tuberculosis
-
-
-
1.1.98.2 Mycobacterium tuberculosis
-
 gene Rv0407
-
1.1.98.2 Mycobacterium tuberculosis H37Rv
-
 gene Rv0407
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.98.2
-
 Mycobacterium tuberculosis
1.1.98.2 recombinant His-tagged wild-type and selenomethionine-labeled FGD1 from Mycobacterium smegmatis strain mc24517 by nickel affinity chromatography and gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.98.2 D-glucose 6-phosphate + oxidized coenzyme F420
-
 Mycobacterium tuberculosis D-glucono-1,5-lactone 6-phosphate + reduced coenzyme F420
-
 ?
1.1.98.2 D-glucose 6-phosphate + oxidized coenzyme F420
-
 Mycobacterium tuberculosis H37Rv D-glucono-1,5-lactone 6-phosphate + reduced coenzyme F420
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.98.2 F420-dependent glucose-6-phosphate dehydrogenase
-
 Mycobacterium tuberculosis
1.1.98.2 FGD1
-
 Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.98.2 coenzyme F420
-
 Mycobacterium tuberculosis

General Information

EC Number General Information Comment Organism
1.1.98.2 physiological function FGD1 is an F420-dependent glucose-6-phosphate dehydrogenase essential for activation of the anti-TB compound PA-824 Mycobacterium tuberculosis