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Literature summary extracted from

  • Yadav, S.; Yadav, P.K.; Yadav, D.; Yadav, K.D.
    Purification and characterization of an alkaline pectin lyase from Aspergillus flavus (2008), Process Biochem., 43, 547-552.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.2.10 Co2+ 25% increase of activity at 1 mM Aspergillus flavus

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.10 Ag+ complete inhibition at 1 mM Aspergillus flavus
4.2.2.10 Ca2+ 88.3% residual activity at 1 mM Aspergillus flavus
4.2.2.10 Cu2+ complete inhibition at 1 mM Aspergillus flavus
4.2.2.10 EDTA 54.2% residual activity at 1 mM Aspergillus flavus
4.2.2.10 Hg2+ complete inhibition at 1 mM Aspergillus flavus
4.2.2.10 K+ 83.3% residual activity at 1 mM Aspergillus flavus
4.2.2.10 Mg2+ 87.5% residual activity at 1 mM Aspergillus flavus
4.2.2.10 additional information not influenced by Mn2+ Aspergillus flavus
4.2.2.10 Na+ 91.6% residual activity at 1 mM Aspergillus flavus
4.2.2.10 potassium ferrocyanide complete inhibition at 1 mM Aspergillus flavus
4.2.2.10 potassium permanganate complete inhibition at 1 mM Aspergillus flavus
4.2.2.10 Sodium arsenate 91.7% residual activity at 1 mM Aspergillus flavus
4.2.2.10 Sodium azide 75.0% residual activity at 1 mM Aspergillus flavus
4.2.2.10 Zn2+ 33.3% residual activity at 1 mM Aspergillus flavus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.10 38000
-
 SDS-PAGE Aspergillus flavus

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.10 Aspergillus flavus
-
-
-
4.2.2.10 Aspergillus flavus MTCC 7589
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.10 ammonium sulfate fractionation, DEAE cellulose chromatography and Sephadex G-100 gel filtration Aspergillus flavus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.2.10 0.316
-
 crude enzyme, in 100 mM sodium phosphate buffer (pH 8.0), at 37°C Aspergillus flavus
4.2.2.10 18.33
-
 after 58.01fold purification, in 100 mM sodium phosphate buffer (pH 8.0), at 37°C Aspergillus flavus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.10 additional information the purified enzyme shows efficacy in retting of Crotalaria juncea fibers Aspergillus flavus ?
-
 ?
4.2.2.10 additional information the purified enzyme shows efficacy in retting of Crotalaria juncea fibers Aspergillus flavus MTCC 7589 ?
-
 ?
4.2.2.10 pectin
-
 Aspergillus flavus 4,5-unsaturated oligogalacturonates
-
 ?
4.2.2.10 pectin
-
 Aspergillus flavus MTCC 7589 4,5-unsaturated oligogalacturonates
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.2.10 PNL
-
 Aspergillus flavus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.10 50
-
-
 Aspergillus flavus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.10 50 70 the enzyme does not loose activity up to 50°C if exposed for 1 h, addition of ammonium sulfate in the range of 0.1-2.0 M increases the thermostability of the enzyme with 0.6 and 1.8 M of ammonium sulfate providing complete stability at 60 and 70°C. respectively, 40% of activity is recovered after incubating the purified enzyme at 60°C for 1 h, while there is 12% of activity recovered after incubation at 70°C for 1 h Aspergillus flavus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.2.10 52.2
-
 Pectin
-
 Aspergillus flavus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.10 8
-
-
 Aspergillus flavus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.2.10 4 10
-
 Aspergillus flavus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.2.2.10 4 10 the enzyme is stable for 24 h in the pH range 4.0-10.0 Aspergillus flavus