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Literature summary extracted from

  • Wu, S.M.; Morris, D.P.; Stafford, D.W.
    Identification and purification to near homogeneity of the vitamin K-dependent carboxylase (1991), Proc. Natl. Acad. Sci. USA, 88, 2236-2240.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.90 FFRCK peptide, protease inhibitor Bos taurus
4.1.1.90 FPRCK peptide, protease inhibitor Bos taurus
4.1.1.90 protease inhibitor mixture PIC, freshly prepared as a 10x PIC stock containing 20 mM dithiothreitol, 20 mM EDTA, FFRCK (1.25 microg/ml), FPRCK (1.25 microg/ml), leupeptin (5 microg/ml), pepstatin A (7 microg/ml), phenylmethylsulfonyl fluoride (340 microg/ml), aprotinin (20 microg/ml) Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.1.1.90 microsome
-
 Bos taurus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.90 94000
-
 SDS-PAGE Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.90 Bos taurus Q07175
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.90 degree of purification is about 7000fold with reference to ammonium sulfate-fractionated microsomal protein from liver. Purification of carboxylase, solubilized microsomes: 281000 cpm/mg/h, flow-through of Affi-FIXQ/S: 200000 cpm/mg/h, bound to Affi-FIXQ/S: 140000000 cpm/mg/h, affinity-purified carboxylase: 1930000000 cpm/mg/h Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.1.90 liver
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.90 FLEEL + CO2 + O2 + vitamin KH2 Phe-Leu-Glu-Glu-Leu Bos taurus ? + vitamin K epoxide + H2O
-
 ?
4.1.1.90 L-glutamate + CO2 + O2 + vitamin K hydroquinone catalyzes modification of specific glutamic acids to gamma-carboxyglutamic acid in several blood-coagulation proteins Bos taurus gamma-carboxy-L-glutamate + vitamin K epoxide + H2O
-
 ?
4.1.1.90 proFIXl9 + CO2 + O2 + vitamin KH2 AVFLDHENANKILNRPKRY Bos taurus ? + vitamin K epoxide + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.90 ? x * 94000, SDS-PAGE Bos taurus

Synonyms

EC Number Synonyms Comment Organism
4.1.1.90 glutamate carboxylase
-
 Bos taurus
4.1.1.90 vitamin K-dependent carboxylase
-
 Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.90 vitamin K
-
 Bos taurus