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Literature summary extracted from

  • Scharte, J.; Schoen, H.; Tjaden, Z.; Weis, E.; von Schaewen, A.
    Isoenzyme replacement of glucose-6-phosphate dehydrogenase in the cytosol improves stress tolerance in plants (2009), Proc. Natl. Acad. Sci. USA, 106, 8061-8066.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.49 additional information after pathogen infection with Phytophthora nicotianae, G6PDH activity rises 2 to 3fold in leaves of a resistant, but not of a susceptible, tobacco cultivar Nicotiana tabacum

Application

EC Number Application Comment Organism
1.1.1.49 additional information because isoenzyme replacement of G6PDH in the cytosol of tobacco is beneficial under various kinds of cues, this strategy may be a tool to enhance stress tolerance in general Arabidopsis thaliana

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.49 into vector pET16b and expressed in a G6PDH-deficient Escherichia coli strain. Overexpression in the cytosol of the susceptible tobacco cultivar Xanthi Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.49 glucosamine 6-phosphate competitive G6PDH inhibitor Nicotiana tabacum
1.1.1.49 NADPH
-
 Arabidopsis thaliana
1.1.1.49 NADPH
-
 Nicotiana tabacum
1.1.1.49 RNAi suppression of endogenous cytosolic G6PDH isoforms result in highly uniform defense responses and also enhanced drought tolerance and flowering Nicotiana tabacum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.49 0.024
-
 NADP+
-
 Arabidopsis thaliana
1.1.1.49 0.041
-
 NADP+ G6PDH activity extracted from source leaves of wild-type tobacco SNN cultivars Nicotiana tabacum
1.1.1.49 0.041
-
 NADP+ G6PDH activity extracted from source leaves of wild-type tobacco Xanthi cultivars Nicotiana tabacum
1.1.1.49 0.24
-
 D-glucose 6-phosphate G6PDH activity extracted from source leaves of wild-type tobacco SNN cultivars Nicotiana tabacum
1.1.1.49 0.25
-
 D-glucose 6-phosphate G6PDH activity extracted from source leaves of wild-type tobacco Xanthi cultivars Nicotiana tabacum
1.1.1.49 0.57
-
 D-glucose 6-phosphate
-
 Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.49 cytosol cytosolic G6PDH seems to be a crucial factor for the outcome of plant defense responses Nicotiana tabacum 5829
-
1.1.1.49 plastid
-
 Arabidopsis thaliana 9536
-

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.49 Arabidopsis thaliana Q8L743
-
-
1.1.1.49 Nicotiana tabacum
-
 var. Samsun, resistant SNN cultivar and susceptible Xanthi cultivar
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.49 leaf
-
 Nicotiana tabacum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.49 D-glucose 6-phosphate + NADP+
-
 Nicotiana tabacum D-glucono-1,5-lactone 6-phosphate + NADPH + H+
-
 ?
1.1.1.49 D-glucose 6-phosphate + NADP+
-
 Arabidopsis thaliana D-glucono-1,5-lactone 6-phosphate + NADPH + H+
-
 ?
1.1.1.49 additional information transgenic (previously susceptible) lines overexpressing G6PDH-encoding gene, displaying high NADPH tolerance engineered for cytosolic expression, show early oxidative bursts, callose deposition, and changes in metabolic parameter Nicotiana tabacum ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.49 G6PDH
-
 Nicotiana tabacum
1.1.1.49 G6PDH
-
 Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.49 NADP+
-
 Nicotiana tabacum
1.1.1.49 NADP+
-
 Arabidopsis thaliana

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.49 0.05
-
 NADPH G6PDH activity extracted from source leaves of wild-type tobacco Xanthi cultivars Nicotiana tabacum
1.1.1.49 0.051
-
 NADPH G6PDH activity extracted from source leaves of wild-type tobacco SNN cultivars Nicotiana tabacum
1.1.1.49 0.06
-
 NADPH
-
 Arabidopsis thaliana