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Literature summary extracted from

  • Auld, D.S.; Thorne, N.; Maguire, W.F.; Inglese, J.
    Mechanism of PTC124 activity in cell-based luciferase assay of nonsense codon suppression (2009), Proc. Natl. Acad. Sci. USA, 106, 3585-3590.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.13.12.5 MS-275
-
 Renilla reniformis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.12.5 expression in HEK-293 cells or Grip-Tite 293 MSR cells Renilla reniformis

Protein Variants

EC Number Protein Variants Comment Organism
1.13.12.5 T329G no significant influence on enzyme activity Renilla reniformis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.12.5 N-benzyl-4-methylbenzenesulfonamide inhibition of purified enzyme, inducing activity in cell-based assay Renilla reniformis

Organism

EC Number Organism UniProt Comment Textmining
1.13.12.5 Renilla reniformis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.12.5
-
 Renilla reniformis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.12.5 coelenterazine-H + O2 5 microM, assay at pH 7.5 Renilla reniformis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.12.5 Renilla reniformis luciferase
-
 Renilla reniformis
1.13.12.5 RLuc
-
 Renilla reniformis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.12.5 7.5
-
 assay at Renilla reniformis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.13.12.5 0.0012
-
 N-benzyl-4-methylbenzenesulfonamide
-
 Renilla reniformis