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Literature summary extracted from

  • Vamecq, J.; de Hoffmann, E.; van Hoof, F.
    The microsomal dicarboxylyl-CoA synthetase (1985), Biochem. J., 230, 683-693.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
6.2.1.23 freezing and thawing of liver homogenates strongly diminishes its activity within several h Rattus norvegicus
6.2.1.23 preincubation of fresh liver homogenate in the absence of substrate and cofactors leads to a loss of 75% of its activity within 30 min Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.2.1.23 AMP product inhibition Rattus norvegicus
6.2.1.23 diphosphate product inhibition Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.23 0.55
-
 ATP
-
 Rattus norvegicus
6.2.1.23 2.5
-
 dodecanedioic acid
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.2.1.23 endoplasmic reticulum
-
 Rattus norvegicus 5783
-
6.2.1.23 microsome
-
 Rattus norvegicus
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.23 additional information Rattus norvegicus the dicarboxylyl-CoA synthetase appears to catalyze an essential step in the degradation of dicarboxylic acids ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.23 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.2.1.23 liver
-
 Rattus norvegicus
-
6.2.1.23 additional information no activity detected in kidney, jejunum, heart, lung, skeletal muscle, smooth muscle, cerebrum, brain stem Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.23 ATP + decanedioic acid + CoA
-
 Rattus norvegicus AMP + diphosphate + decanedioyl-CoA
-
 ?
6.2.1.23 ATP + dodec-2-enedioic acid + CoA i.e. traumatic acid, at 40% of the activity relative to dodecanedioic acid Rattus norvegicus AMP + diphosphate + dodec-2-enedioyl-CoA
-
 ?
6.2.1.23 ATP + dodecanedioic acid + CoA highest activity with dodecandioic acid , ATP cannot be replaced by GTP Rattus norvegicus AMP + diphosphate + dodecanedioyl-CoA
-
 ?
6.2.1.23 ATP + heptanedioic acid + CoA
-
 Rattus norvegicus AMP + diphosphate + heptanedioyl-CoA
-
 ?
6.2.1.23 ATP + hexadecanedioic acid + CoA
-
 Rattus norvegicus AMP + diphosphate + hexadecanedioyl-CoA
-
 ?
6.2.1.23 ATP + hexanedioic acid + CoA
-
 Rattus norvegicus AMP + diphosphate + hexanedioyl-CoA
-
 ?
6.2.1.23 ATP + octanedioic acid + CoA
-
 Rattus norvegicus AMP + diphosphate + octanedioyl-CoA
-
 ?
6.2.1.23 ATP + pentanedioic acid + CoA
-
 Rattus norvegicus AMP + diphosphate + pentanedioyl-CoA
-
 ?
6.2.1.23 ATP + tetradecanedioic acid + CoA
-
 Rattus norvegicus AMP + diphosphate + tetradecanedioyl-CoA
-
 ?
6.2.1.23 additional information the dicarboxylyl-CoA synthetase appears to catalyze an essential step in the degradation of dicarboxylic acids Rattus norvegicus ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.23 6.5
-
 dodecanedioic acid Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.2.1.23 7.4 8.5 pH 7.4: 40% of maximal activity, pH 8.5: 70% of maximal activity with dodecanedioic acid Rattus norvegicus