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Literature summary extracted from

  • Shah, N.; Kuntz, D.A.; Rose, D.R.
    Golgi alpha-mannosidase II cleaves two sugars sequentially in the same catalytic site (2008), Proc. Natl. Acad. Sci. USA, 105, 9570-9575.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.114 in complex with natural substrate GlcNAcMan5GlcNAc2 and with oligosaccharide Man5. The natural substrate binds in a large groove on the surface of the enzyme. This groove contains the site of the nucleophile D204, acid/base catalyst D341, and zinc ion. substrate binding does not induce any noticeable conformational change. the larger active site cleft contains three sugar-binding subsites Drosophila melanogaster

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.114 Golgi apparatus
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 Drosophila melanogaster 5794
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Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.114 Zn2+ crystallization data Drosophila melanogaster

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.114 Drosophila melanogaster Q24451 isoform GMII
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.114 2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1->2)-alpha-D-mannopyranosyl-(1->3)-[alpha-D-mannopyranosyl-(1->3)-alpha-D-mannopyranosyl-(1->6)]-beta-D-mannopyranosyl-(1->4)-2-(acetylamino)-2-deoxy-alpha-D-glucopyranose + H2O
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 Drosophila melanogaster 2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1->2)-alpha-D-mannopyranosyl-(1->3)-[alpha-D-mannopyranosyl-(1->6)]-beta-D-mannopyranosyl-(1->4)-2-(acetylamino)-2-deoxy-alpha-D-glucopyranose + D-mannose
-
 ir
3.2.1.114 2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1->2)-alpha-D-mannopyranosyl-(1->3)-[alpha-D-mannopyranosyl-(1->3)-[alpha-D-mannopyranosyl-(1->6)]-alpha-D-mannopyranosyl-(1->6)]-beta-D-mannopyranosyl-(1->4)-2-(acetylamino)-2-deoxy-alpha-D-glucopyranose + H2O
-
 Drosophila melanogaster 2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1->2)-alpha-D-mannopyranosyl-(1->3)-[alpha-D-mannopyranosyl-(1->3)-alpha-D-mannopyranosyl-(1->6)]-beta-D-mannopyranosyl-(1->4)-2-(acetylamino)-2-deoxy-alpha-D-glucopyranose + D-mannose
-
 ir