EC Number | Cloned (Comment) | Organism |
---|---|---|
1.13.12.17 | the coding region for the enzyme component StaP is cloned into expression vector pET26b(+) (Novagen, San Diego, CA), with a C-terminal 6 * His tag, and overexpressed in Escherichia coli strain BL21 | Streptomyces sp. |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.13.12.17 | X-ray crystal structures of chromopyrrolic acid-bound and chromopyrrolic acid-free forms of enzyme component StaP. The enzyme attains a more ordered conformation upon binding of the substrate. Substantial conformational changes are observed at and around the substrate-binding pocket to optimize interactions with the enzyme. The enzymechromopyrrolic acid complex structure reveals that chromopyrrolic acid has a twisted-butterfly shape in the active site, which is perpendicular to the heme plane | Streptomyces sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.12.17 | dichlorochromopyrrolate + 4 O2 + 4 NADH + 4 H+ | Streptomyces sp. | staurosporine biosynthesis | dichloroarcyriaflavin A + 2 CO2 + 6 H2O + 4 NAD+ | - |
? | |
1.13.12.17 | dichlorochromopyrrolate + 4 O2 + 4 NADH + 4 H+ | Streptomyces sp. TP-A0274 | staurosporine biosynthesis | dichloroarcyriaflavin A + 2 CO2 + 6 H2O + 4 NAD+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.13.12.17 | Streptomyces sp. | Q83WG3 | TP-A0274 | - |
1.13.12.17 | Streptomyces sp. TP-A0274 | Q83WG3 | TP-A0274 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.13.12.17 | - |
Streptomyces sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.12.17 | dichlorochromopyrrolate + 4 O2 + 4 NADH + 4 H+ | - |
Streptomyces sp. | dichloroarcyriaflavin A + 2 CO2 + 6 H2O + 4 NAD+ | - |
? | |
1.13.12.17 | dichlorochromopyrrolate + 4 O2 + 4 NADH + 4 H+ | staurosporine biosynthesis | Streptomyces sp. | dichloroarcyriaflavin A + 2 CO2 + 6 H2O + 4 NAD+ | - |
? | |
1.13.12.17 | dichlorochromopyrrolate + 4 O2 + 4 NADH + 4 H+ | - |
Streptomyces sp. TP-A0274 | dichloroarcyriaflavin A + 2 CO2 + 6 H2O + 4 NAD+ | - |
? | |
1.13.12.17 | dichlorochromopyrrolate + 4 O2 + 4 NADH + 4 H+ | staurosporine biosynthesis | Streptomyces sp. TP-A0274 | dichloroarcyriaflavin A + 2 CO2 + 6 H2O + 4 NAD+ | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.13.12.17 | CYP245A1 | - |
Streptomyces sp. |
1.13.12.17 | cytochrome P450 StaP | - |
Streptomyces sp. |
1.13.12.17 | StaP | - |
Streptomyces sp. |