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Literature summary extracted from

  • Badr, C.; Hewett, J.; Breakefield, X.; Tannous, B.
    A highly sensitive assay for monitoring the secretory pathway and ER stress (2007), PLoS ONE, 6, e571.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.13.12.5 additional information Gluc reporter as a sensitive marker in elucidation endoplasmic reticulum stress in mammalian cells Gaussia princeps
1.13.12.5 additional information monitor processing of proteins through the secretory pathway and endoplasmic reticulum Gaussia princeps

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.12.5 expression in 293T HEK fibroblasts and HF8 human fibroblasts Gaussia princeps

Protein Variants

EC Number Protein Variants Comment Organism
1.13.12.5 additional information fusion of Gluc to fluorescent protein YFP Gaussia princeps

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.12.5 brefeldinA 5 microg/ml, 24 h, 90% decrease of Gluc secretion Gaussia princeps
1.13.12.5 cytochalasin B 5 microg7ml, 24 h, 30% decrease of Gluc secretion Gaussia princeps
1.13.12.5 dithiothreitol 1 mM, complete inhibition of secretion for the first 3 h, secretion returned to normal level after 24 h Gaussia princeps
1.13.12.5 monensin A 3 microg/ml, 24 h, 65% decrease of Gluc secretion Gaussia princeps
1.13.12.5 nocodazole 10 microg/ml, 24 h, 75% decrease of Gluc secretion Gaussia princeps
1.13.12.5 thapsigargin 0.03 microM, about 30% decrease of Gluc secretion and activity Gaussia princeps
1.13.12.5 tunicamycin
-
 Gaussia princeps

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.13.12.5 endoplasmic reticulum
-
 Gaussia princeps 5783
-
1.13.12.5 additional information secreted Gaussia princeps
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.12.5 47000
-
 Western blot, fusion protein Gluc-YFP Gaussia princeps

Organism

EC Number Organism UniProt Comment Textmining
1.13.12.5 Gaussia princeps
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.12.5 beetle D-luciferin 450 microM Gaussia princeps ?
-
 ?
1.13.12.5 coelenterazine + ? 20 microM Gaussia princeps ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.12.5 firefly luciferase
-
 Gaussia princeps
1.13.12.5 fluc
-
 Gaussia princeps
1.13.12.5 Gaussia luciferase
-
 Gaussia princeps