Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Herbst, K.J.; Allen, M.D.; Zhang, J.
    The cAMP-dependent protein kinase inhibitor H-89 attenuates the bioluminescence signal produced by renilla luciferase (2009), Plos One, 4, e5642.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.13.12.5 pharmacology investigations into regulation and functional roles of kinases Renilla sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.12.5 expressed in HEK-293T cells Renilla sp.
1.13.12.5 expression of RLuc8 in Escherichia coli BL21 Renilla sp.

Protein Variants

EC Number Protein Variants Comment Organism
1.13.12.5 RLuc8 eight-point mutation variant of renilla luciferase Renilla sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.12.5 H-89 10 microM, reduced luminescence to 50% of RLuc and to 54% of RLuc8 Renilla sp.

Organism

EC Number Organism UniProt Comment Textmining
1.13.12.5 Renilla sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.12.5
-
 Renilla sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.12.5 b-coelenterazine + O2
-
 Renilla sp. ?
-
 ?
1.13.12.5 D-luciferin + O2
-
 Renilla sp. ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.12.5 firefly luciferase
-
 Renilla sp.
1.13.12.5 fluc
-
 Renilla sp.
1.13.12.5 Renilla luciferase
-
 Renilla sp.
1.13.12.5 RLuc
-
 Renilla sp.
1.13.12.5 RLuc8 eight-point mutation variant of RLuc Renilla sp.

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.13.12.5 0.021
-
 10 microM coelenterazine, measured RLuc8 activity Renilla sp. H-89