BRENDA - Enzyme Database

Purification and characterization of hydroxycinnamoyl-coenzyme A:omega-hydroxypalmitic acid O-hydroxycinnamoyltransferase from tobacco (Nicotiana tabacum) cell-suspension cultures

Lotfy, S.; Javelle, F.; Negrel, J.; Planta 199, 475-480 (1996)
No PubMed abstract available

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.188
0.0003
-
16-hydroxypalmitic acid
pH 7.0, 30°C
Nicotiana tabacum
2.3.1.188
0.0007
-
hexadecan-1,16-diol
pH 7.0, 30°
Nicotiana tabacum
2.3.1.188
0.001
-
1-Tetradecanol
pH 7.0, 30°C
Nicotiana tabacum
2.3.1.188
0.002
-
1-hexadecanol
pH 7.0, 30°C
Nicotiana tabacum
2.3.1.188
0.002
-
1-Dodecanol
pH 7.0, 30°C
Nicotiana tabacum
2.3.1.188
0.0033
-
sinapoyl-CoA
pH 7.0, 30°C
Nicotiana tabacum
2.3.1.188
0.01
-
p-Coumaroyl-CoA
pH 7.0, 30°C
Nicotiana tabacum
2.3.1.188
0.0112
-
15-hydroxypentadecanoic acid
pH 7.0, 30°C
Nicotiana tabacum
2.3.1.188
0.015
-
feruloyl-CoA
pH 7.0, 30°C
Nicotiana tabacum
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.188
55000
-
x * 55000, SDS-PAGE
Nicotiana tabacum
Organism
EC Number
Organism
UniProt
Commentary
Textmining
2.3.1.188
Nicotiana tabacum
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
2.3.1.188
-
Nicotiana tabacum
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.188
cell suspension culture
late logarithmic phase
Nicotiana tabacum
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2.3.1.188
feruloyl-CoA + 1-dodecanol
49% of the activity with 16-hydroxypalmitic acid
700854
Nicotiana tabacum
CoA + (E)-dodecyl 3-(4-hydroxy-3-methoxyphenyl)acrylate
-
-
-
?
2.3.1.188
feruloyl-CoA + 1-hexadecanol
-
700854
Nicotiana tabacum
CoA + (E)-hexadecyl 3-(4-hydroxy-3-methoxyphenyl)acrylate
-
-
-
?
2.3.1.188
feruloyl-CoA + 1-pentadecanol
53% of the activity with 16-hydroxypalmitic acid
700854
Nicotiana tabacum
CoA + (E)-pentadecyl 3-(4-hydroxy-3-methoxyphenyl)acrylate
-
-
-
?
2.3.1.188
feruloyl-CoA + 1-tetradecanol
82% of the activity with 16-hydroxypalmitic acid
700854
Nicotiana tabacum
CoA + (E)-tetradecyl 3-(4-hydroxy-3-methoxyphenyl)acrylate
-
-
-
?
2.3.1.188
feruloyl-CoA + 15-hydroxypentadecanoic acid
68% of the activity with 16-hydroxypalmitate
700854
Nicotiana tabacum
CoA + (E)-15-(3-(4-hydroxy-3-methoxyphenyl)acryloyloxy)pentadecanoic acid
-
-
-
?
2.3.1.188
feruloyl-CoA + 16-hydroxypalmitic acid
-
700854
Nicotiana tabacum
CoA + (E)-16-(3-(4-hydroxy-3-methoxyphenyl)acryloyloxy)hexadecanoic acid
-
-
-
?
2.3.1.188
feruloyl-CoA + hexadecane-1,16-diol
52% of the activity with 16-hydroxypalmitic acid
700854
Nicotiana tabacum
CoA + (E)-16-hydroxyhexadecyl 3-(4-hydroxy-3-methoxyphenyl)acrylate
-
-
-
?
2.3.1.188
additional information
no substrate: 18-hydroxy-cis-9-octadecenoic acid
700854
Nicotiana tabacum
?
-
-
-
?
2.3.1.188
p-coumaroyl-CoA + 16-hydroxypalmitic acid
51% of the activity with feruloyl-CoA
700854
Nicotiana tabacum
CoA + (E)-16-(3-(4-hydroxyphenyl)acryloyloxy)hexadecanoic acid
-
-
-
?
2.3.1.188
sinapoyl-CoA + 16-hydroxypalmitic acid
50% of the activity with feruloyl-CoA
700854
Nicotiana tabacum
CoA + (E)-16-(3-(4-hydroxy-3,5-dimethoxyphenyl)acryloyloxy)hexadecanoic acid
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.3.1.188
?
x * 55000, SDS-PAGE
Nicotiana tabacum
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
2.3.1.188
Nicotiana tabacum
isoelectric focusing
-
4.6
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.188
0.0003
-
16-hydroxypalmitic acid
pH 7.0, 30°C
Nicotiana tabacum
2.3.1.188
0.0007
-
hexadecan-1,16-diol
pH 7.0, 30°
Nicotiana tabacum
2.3.1.188
0.001
-
1-Tetradecanol
pH 7.0, 30°C
Nicotiana tabacum
2.3.1.188
0.002
-
1-hexadecanol
pH 7.0, 30°C
Nicotiana tabacum
2.3.1.188
0.002
-
1-Dodecanol
pH 7.0, 30°C
Nicotiana tabacum
2.3.1.188
0.0033
-
sinapoyl-CoA
pH 7.0, 30°C
Nicotiana tabacum
2.3.1.188
0.01
-
p-Coumaroyl-CoA
pH 7.0, 30°C
Nicotiana tabacum
2.3.1.188
0.0112
-
15-hydroxypentadecanoic acid
pH 7.0, 30°C
Nicotiana tabacum
2.3.1.188
0.015
-
feruloyl-CoA
pH 7.0, 30°C
Nicotiana tabacum
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.188
55000
-
x * 55000, SDS-PAGE
Nicotiana tabacum
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.188
-
Nicotiana tabacum
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.188
cell suspension culture
late logarithmic phase
Nicotiana tabacum
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2.3.1.188
feruloyl-CoA + 1-dodecanol
49% of the activity with 16-hydroxypalmitic acid
700854
Nicotiana tabacum
CoA + (E)-dodecyl 3-(4-hydroxy-3-methoxyphenyl)acrylate
-
-
-
?
2.3.1.188
feruloyl-CoA + 1-hexadecanol
-
700854
Nicotiana tabacum
CoA + (E)-hexadecyl 3-(4-hydroxy-3-methoxyphenyl)acrylate
-
-
-
?
2.3.1.188
feruloyl-CoA + 1-pentadecanol
53% of the activity with 16-hydroxypalmitic acid
700854
Nicotiana tabacum
CoA + (E)-pentadecyl 3-(4-hydroxy-3-methoxyphenyl)acrylate
-
-
-
?
2.3.1.188
feruloyl-CoA + 1-tetradecanol
82% of the activity with 16-hydroxypalmitic acid
700854
Nicotiana tabacum
CoA + (E)-tetradecyl 3-(4-hydroxy-3-methoxyphenyl)acrylate
-
-
-
?
2.3.1.188
feruloyl-CoA + 15-hydroxypentadecanoic acid
68% of the activity with 16-hydroxypalmitate
700854
Nicotiana tabacum
CoA + (E)-15-(3-(4-hydroxy-3-methoxyphenyl)acryloyloxy)pentadecanoic acid
-
-
-
?
2.3.1.188
feruloyl-CoA + 16-hydroxypalmitic acid
-
700854
Nicotiana tabacum
CoA + (E)-16-(3-(4-hydroxy-3-methoxyphenyl)acryloyloxy)hexadecanoic acid
-
-
-
?
2.3.1.188
feruloyl-CoA + hexadecane-1,16-diol
52% of the activity with 16-hydroxypalmitic acid
700854
Nicotiana tabacum
CoA + (E)-16-hydroxyhexadecyl 3-(4-hydroxy-3-methoxyphenyl)acrylate
-
-
-
?
2.3.1.188
additional information
no substrate: 18-hydroxy-cis-9-octadecenoic acid
700854
Nicotiana tabacum
?
-
-
-
?
2.3.1.188
p-coumaroyl-CoA + 16-hydroxypalmitic acid
51% of the activity with feruloyl-CoA
700854
Nicotiana tabacum
CoA + (E)-16-(3-(4-hydroxyphenyl)acryloyloxy)hexadecanoic acid
-
-
-
?
2.3.1.188
sinapoyl-CoA + 16-hydroxypalmitic acid
50% of the activity with feruloyl-CoA
700854
Nicotiana tabacum
CoA + (E)-16-(3-(4-hydroxy-3,5-dimethoxyphenyl)acryloyloxy)hexadecanoic acid
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.3.1.188
?
x * 55000, SDS-PAGE
Nicotiana tabacum
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
2.3.1.188
Nicotiana tabacum
isoelectric focusing
-
4.6