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Literature summary extracted from

  • Sattler, S.E.; Saathoff, A.J.; Haas, E.J.; Palmer, N.A.; Funnell-Harris, D.L.; Sarath, G.; Pedersen, J.F.
    A nonsense mutation in a cinnamyl alcohol dehydrogenase gene is responsible for the Sorghum brown midrib6 phenotype (2009), Plant Physiol., 150, 584-595.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.195 entire coding regions of CAD4 cloned into the pET30a vector and expressed in Escherichia coli Rossetta R2 cells Sorghum bicolor
1.1.1.195 expression in Escherichia coli Sorghum bicolor

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.195 Q132Stop mutation responsible for the bmr6 phenotype. Mutation leads to significant reduction in all three main lignin subunits, H-, G-, and S-lignin of 4.8-, 7.3-, and 17.7fold, respectively, relative to the wild type. Lignin subunits S-indene and G-indene are elevated 9.5- and 8.3fold, respectively, in bmr6 relative to the wild type Sorghum bicolor

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.195 0.0075
-
 coniferyl alcohol
-
 Sorghum bicolor
1.1.1.195 0.0075
-
 coniferyl alcohol pH 8.8, temperature not specified in the publication Sorghum bicolor
1.1.1.195 0.117
-
 coniferyl alcohol
-
 Sorghum bicolor
1.1.1.195 0.117
-
 coniferyl alcohol pH 8.8, temperature not specified in the publication Sorghum bicolor

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.195 Sorghum bicolor
-
-
-
1.1.1.195 Sorghum bicolor A1ILL4 isoform CAD2
-
1.1.1.195 Sorghum bicolor C5XC49
-
-
1.1.1.195 Sorghum bicolor C5XC49 isoform CAD4
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.195 by affinity purification on a nickel column Sorghum bicolor
1.1.1.195 recombinant enzyme Sorghum bicolor

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.195 internode Bmr6 expression in young internodes is significantly higher than CAD4 for all genotypes Sorghum bicolor
-
1.1.1.195 internode Bmr6 expression in young internodes is significantly higher than CAD4 for all genotypes, but only 4fold to 5fold higher in bmr6 and bmr6 bmr12 internodes. Bmr6 expression is significantly decreased in bmr6 and bmr6 bmr12 compared to the wild-type and bmr12, 20fold and 15fold, respectively Sorghum bicolor
-
1.1.1.195 additional information polymorphic restriction site is present in the near-isogenic lines Atlas bmr6, RTx430 bmr6, and Wheatland bmr6 and is absent from wild-type Atlas, RTx430, and Wheatland Sorghum bicolor
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.195 benzyl alcohol + NADP+ CAD4 displays slight activity for benzoyl alcohol Sorghum bicolor benzaldehyde + NADPH + H+
-
 ?
1.1.1.195 benzyl alcohol + NADP+ slight activity Sorghum bicolor benzylaldehyde + NADPH + H+
-
 r
1.1.1.195 coniferyl alcohol + NADP+
-
 Sorghum bicolor coniferyl aldehyde + NADPH + H+
-
 r
1.1.1.195 coniferyl alcohol + NADP+ Bmr6 displays significantly greater activity in comparison to CAD4. Activity of Bmr6 is 2.5- and 34fold higher than CAD4 for coniferyl and sinapyl aldehyde, respectively Sorghum bicolor coniferyl aldehyde + NADPH + H+
-
 ?
1.1.1.195 coniferyl alcohol + NADP+ Bmr6 displays significantly greater activity in comparison to CAD4. Bmr6 activity is 2.2- and 2.6-fold greater respectively, when coumaryl and sinapyl alcohols are used as substrates. Activity of Bmr6 is 2.5- and 34fold higher than CAD4 for coniferyl and sinapyl aldehyde, respectively Sorghum bicolor coniferyl aldehyde + NADPH + H+
-
 ?
1.1.1.195 coniferyl alcohol + NADP+ Bmr6 activity is 2.2- and 2.6fold greater respectively, when coumaryl and sinapyl alcohols are used as substrates compared to coniferyl alcohol as a substrate Sorghum bicolor coniferyl aldehyde + NADPH + H+
-
 r
1.1.1.195 coniferyl aldehyde + NADPH + H+ best substrate Sorghum bicolor coniferyl alcohol + NADP+
-
 r
1.1.1.195 coniferyl aldehyde + NADPH + H+ Brm6 has higher activities for the coniferyl and sinapyl aldehydes in comparison to the corresponding alcohols Sorghum bicolor coniferyl alcohol + NADP+
-
 r
1.1.1.195 coumaryl alcohol + NADP+
-
 Sorghum bicolor coumaryl aldehyde + NADPH + H+
-
 r
1.1.1.195 coumaryl alcohol + NADP+ Bmr6 displays significantly greater activity in comparison to CAD4 Sorghum bicolor coumaryl aldehyde + NADPH + H+
-
 ?
1.1.1.195 coumaryl alcohol + NADP+ Bmr6 activity is 2.2- and 2.6fold greater respectively, when coumaryl and sinapyl alcohols are used as substrates compared to coniferyl alcohol as a substrate Sorghum bicolor coumaryl aldehyde + NADPH + H+
-
 r
1.1.1.195 additional information a C-to-T transition mutation is present in bmr6 but not in any wild-type sequence. This mutation changes amino acid 132 of the protein from Gln (CAG) to a stop codon (UAG). The bmr6 sequence encodes a predicted truncated protein that lacks the nucleotide binding (NADPH) and C-terminal catalytic domains, thus bmr6 is presumably a null allele. No activity with caffeoyl alcohol and benzoyl alcohol Sorghum bicolor ?
-
 ?
1.1.1.195 additional information CAD4 expression is significantly increased in bmr6 and bmr6 bmr12 plants relative to the wild-type and bmr12, 5- and 2.5fold, respectively, which may highlight a compensatory mechanism for loss of CAD activity in bmr6. No activity with caffeoyl alcohol Sorghum bicolor ?
-
 ?
1.1.1.195 sinapyl alcohol + NADP+
-
 Sorghum bicolor sinapyl aldehyde + NADPH + H+
-
 r
1.1.1.195 sinapyl alcohol + NADP+ Bmr6 displays significantly greater activity in comparison to CAD4 Sorghum bicolor sinapyl aldehyde + NADPH + H+
-
 ?
1.1.1.195 sinapyl alcohol + NADP+ Bmr6 activity is 2.2- and 2.6fold greater respectively, when coumaryl and sinapyl alcohols are used as substrates compared to coniferyl alcohol as a substrate Sorghum bicolor sinapyl aldehyde + NADPH + H+
-
 r
1.1.1.195 sinapyl aldehyde + NADPH + H+
-
 Sorghum bicolor sinapyl alcohol + NADP+
-
 r
1.1.1.195 sinapyl aldehyde + NADPH + H+ Brm6 has higher activities for the coniferyl and sinapyl aldehydes in comparison to the corresponding alcohols Sorghum bicolor sinapyl alcohol + NADP+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.195 Bmr6
-
 Sorghum bicolor
1.1.1.195 brown midrib6
-
 Sorghum bicolor
1.1.1.195 CAD2
-
 Sorghum bicolor
1.1.1.195 CAD4
-
 Sorghum bicolor
1.1.1.195 cinnamyl alcohol dehydrogenase
-
 Sorghum bicolor