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Literature summary extracted from

  • Zhao, H.; Dong, J.; Wang, T.
    Function and expression analysis of gibberellin oxidases in apple (2009), Plant Mol. Biol. Rep., 28, 231-238.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.11.13 rapid amplification of cDNA ends, expression vector produced by PCR, inserted into PMD 18 T-Simple Vector, inserted into pMAL-c2x vector, transformed and expressed in Escherichia coli Tb1 Malus domestica

Protein Variants

EC Number Protein Variants Comment Organism
1.14.11.13 additional information fusion enzyme MdGA2ox1-MBP Malus domestica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.11.13 additional information
-
 1339 bp, 1014 open reading frame encodes a putative protein of 334 amino acids Malus domestica

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.13 Malus domestica
-
 cultivar Fuji on rootstock Malus prunifolia
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.11.13 Escherichia coli cells with recombinant enzyme grown at 37°C, harvested and suspended in lysis buffer (100 mM Tris-HCl, pH 7.5), sonication in ice water, centrifuged, supernatant collected and stored at -80°C for enzyme activity assays Malus domestica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.11.13 flower highest expression Malus domestica
-
1.14.11.13 flower bud low expression Malus domestica
-
1.14.11.13 seed relatively strong expression in immature and low expression in mature seeds Malus domestica
-
1.14.11.13 shoot very low expression in apical shoot Malus domestica
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.13 gibberellin 1 + 2-oxoglutarate + O2 100 mM Tris-HCl, pH 7.5, 5 mM 2-oxoglutarate, 5 mM ascorbate, 0.5 mM FeSO4, overnight incubation with 2H2-labeled substrates at 30°C Malus domestica gibberellin 8 + succinate + CO2 + H2O
-
 ?
1.14.11.13 gibberellin 4 + 2-oxoglutarate + O2 100 mM Tris-HCl, pH 7.5, 5 mM 2-oxoglutarate, 5 mM ascorbate, 0.5 mM FeSO4, overnight incubation with 2H2-labeled substrates at 30°C Malus domestica gibberellin 34 + succinate + CO2 + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.11.13 gibberellin 2-oxidase
-
 Malus domestica
1.14.11.13 MdGA2ox1 class one using C19-gibberellins as substrates Malus domestica