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Literature summary extracted from

  • Woo, J.; v. Arnim, A.G.
    Mutational optimization of the coelenterazine-dependent luciferase from Renilla (2008), Plant Methods, 4, 23.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.13.12.5 biotechnology popular reporter enzyme for gene expression and biosensor applications Renilla reniformis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.12.5 expression in Arabidopsis Renilla reniformis
1.13.12.5 expression in Escherichia coli strain BL21 Renilla reniformis

Protein Variants

EC Number Protein Variants Comment Organism
1.13.12.5 K189V increased activity Renilla reniformis
1.13.12.5 M185G slightly increased half life Renilla reniformis
1.13.12.5 M185V increased activity Renilla reniformis
1.13.12.5 P220G only 4% of the initial luciferase activity of wild-type luciferase Renilla reniformis
1.13.12.5 P220L only 16% of the initial luciferase activity of wild-type luciferase Renilla reniformis
1.13.12.5 V267I increased activity Renilla reniformis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.12.5 coelenteramide
-
 Renilla reniformis
1.13.12.5 additional information loss of enzymatic activity when in contact with substrate Renilla reniformis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.13.12.5 cytosol
-
 Renilla reniformis 5829
-

Organism

EC Number Organism UniProt Comment Textmining
1.13.12.5 Renilla reniformis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.12.5
-
 Renilla reniformis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.12.5 coelenterazine + O2 assay at pH 7.2 Renilla reniformis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.12.5 Renilla luciferase
-
 Renilla reniformis
1.13.12.5 RLuc
-
 Renilla reniformis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.12.5 additional information
-
 coelenterazine value 111 micromol/min/micromol enzyme Renilla reniformis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.12.5 7.2
-
 assay at Renilla reniformis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.13.12.5 0.000022
-
 coelenteramide
-
 Renilla reniformis