Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Masuda, S.; Ikeda, R.; Masuda, T.; Hashimoto, H.; Tsuchiya, T.; Kojima, H.; Nomata, J.; Fujita, Y.; Mimuro, M.; Ohta, H.; Takamiya, K.
    Prolamellar bodies formed by cyanobacterial protochlorophyllide oxidoreductase in Arabidopsis (2009), Plant J., 58, 952-960.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.33 expression in Escherichia coli cells Synechocystis sp. PCC 6803
1.3.1.33 expression in Escherichia coli cells Gloeobacter violaceus
1.3.1.33 yes into the vector pTYB for expression in Escherichia coli BL21DE3 cells Arabidopsis thaliana
1.3.1.33 yes into the vector pTYB for expression in Escherichia coli BL21DE3 cells, for the construction of transgenic plants pDONR/ZeO and pGWB2 vectors are used Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.33 0.00015
-
 protochlorophyllide
-
 Arabidopsis thaliana
1.3.1.33 0.00039
-
 protochlorophyllide
-
 Arabidopsis thaliana
1.3.1.33 0.0005
-
 protochlorophyllide
-
 Gloeobacter violaceus
1.3.1.33 0.0075
-
 NADPH
-
 Synechocystis sp. PCC 6803
1.3.1.33 0.01083
-
 protochlorophyllide
-
 Synechocystis sp. PCC 6803
1.3.1.33 0.0196
-
 NADPH
-
 Gloeobacter violaceus
1.3.1.33 0.0268
-
 NADPH
-
 Arabidopsis thaliana
1.3.1.33 0.0595
-
 NADPH
-
 Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.1.33 chloroplast
-
 Arabidopsis thaliana 9507
-
1.3.1.33 etioplast prolamellar body
-
 Arabidopsis thaliana 9541
-
1.3.1.33 plastid
-
 Arabidopsis thaliana 9536
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.33 protochlorophyllide + NADPH + H+ Synechocystis sp. PCC 6803
-
 chlorophyllide + NADP+
-
 ?
1.3.1.33 protochlorophyllide + NADPH + H+ Gloeobacter violaceus
-
 chlorophyllide + NADP+
-
 ?
1.3.1.33 protochlorophyllide + NADPH + H+ Arabidopsis thaliana
-
 chlorophyllide + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.33 Arabidopsis thaliana O48741
-
-
1.3.1.33 Arabidopsis thaliana P21218
-
-
1.3.1.33 Arabidopsis thaliana Q42536
-
-
1.3.1.33 Gloeobacter violaceus
-
-
-
1.3.1.33 Synechocystis sp. PCC 6803
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.33 using a chitin bead column, the chitin-intein tag is removed Arabidopsis thaliana
1.3.1.33 using His-Bind resin Synechocystis sp. PCC 6803
1.3.1.33 using His-Bind resin Gloeobacter violaceus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.33 protochlorophyllide + NADPH + H+
-
 Synechocystis sp. PCC 6803 chlorophyllide + NADP+
-
 ?
1.3.1.33 protochlorophyllide + NADPH + H+
-
 Gloeobacter violaceus chlorophyllide + NADP+
-
 ?
1.3.1.33 protochlorophyllide + NADPH + H+
-
 Arabidopsis thaliana chlorophyllide + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.1.33 POR
-
 Synechocystis sp. PCC 6803
1.3.1.33 POR
-
 Gloeobacter violaceus
1.3.1.33 POR
-
 Arabidopsis thaliana
1.3.1.33 PORA
-
 Arabidopsis thaliana
1.3.1.33 PORB
-
 Arabidopsis thaliana
1.3.1.33 PORC
-
 Arabidopsis thaliana
1.3.1.33 protochlorophyllide oxidoreductase
-
 Synechocystis sp. PCC 6803
1.3.1.33 protochlorophyllide oxidoreductase
-
 Gloeobacter violaceus
1.3.1.33 protochlorophyllide oxidoreductase
-
 Arabidopsis thaliana
1.3.1.33 protochlorophyllide oxidoreductase A
-
 Arabidopsis thaliana
1.3.1.33 protochlorophyllide oxidoreductase B
-
 Arabidopsis thaliana
1.3.1.33 protochlorophyllide oxidoreductase C
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.33 23
-
 activity assay at room temperature Synechocystis sp. PCC 6803
1.3.1.33 23
-
 activity assay at room temperature Gloeobacter violaceus
1.3.1.33 23
-
 activity assay at room temperature Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.33 7.5
-
 activity assay Synechocystis sp. PCC 6803
1.3.1.33 7.5
-
 activity assay Gloeobacter violaceus
1.3.1.33 7.5
-
 activity assay Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.33 NADPH
-
 Synechocystis sp. PCC 6803
1.3.1.33 NADPH
-
 Gloeobacter violaceus
1.3.1.33 NADPH
-
 Arabidopsis thaliana