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Literature summary extracted from

  • Jung, B.; Floerchinger, M.; Kunz, H.H.; Traub, M.; Wartenberg, R.; Jeblick, W.; Neuhaus, H.E.; Moehlmann, T.
    Uridine-ribohydrolase is a key regulator in the uridine degradation pathway of Arabidopsis (2009), Plant Cell, 21, 876-891.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.2.2 URH1, DNA and amino acid sequence determination and analysis, expression in Escherichia coli, functional complementation of a yeast mutant, expression in transgenic Arabidopsis thaliana mutant plants under control of the CaMV 35S promoter Arabidopsis thaliana
3.2.2.3 functional expression of URH1 in Arabidopsis thaliana plants under control of the CaMV 35S promoter Oryza sativa
3.2.2.3 URH1, DNA and amino acid sequence determination and analysis, expression in Escherichia coli, functional complementation of a yeast mutant, expression in transgenic Arabidopsis thaliana mutant plants under control of the CaMV 35S promoter Arabidopsis thaliana
3.2.2.7 URH1, DNA and amino acid sequence determination and analysis, expression in Escherichia coli, functional complementation of a yeast mutant, expression in transgenic Arabidopsis thaliana mutant plants under control of the CaMV 35S promoter Arabidopsis thaliana
3.2.2.8 expressed in Escherichia coli and Saccharomyces cerevisiae Arabidopsis thaliana
3.2.2.8 URH1, DNA and amino acid sequence determination and analysis, expression in Escherichia coli, functional complementation of a yeast mutant, expression in transgenic Arabidopsis thaliana mutant plants under control of the CaMV 35S promoter Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
3.2.2.2 additional information mutants expressing the Arabidopsis thaliana enzyme or the homologue from Oryza sativa exhibit resistance toward toxic fluorouridine, fluorouracil, and fluoroorotic acid. Mutants with increased and decreased nucleosidase activity are delayed in germination Arabidopsis thaliana
3.2.2.3 additional information mutants expressing the Arabidopsis thaliana enzyme or the homologue from Oryza sativa exhibit resistance toward toxic fluorouridine, fluorouracil, and fluoroorotic acid. Mutants with increased and decreased nucleosidase activity are delayed in germination Arabidopsis thaliana
3.2.2.7 additional information mutants expressing the Arabidopsis thaliana enzyme or the homologue from Oryza sativa exhibit resistance toward toxic fluorouridine, fluorouracil, and fluoroorotic acid. Mutants with increased and decreased nucleosidase activity are delayed in germination Arabidopsis thaliana
3.2.2.8 additional information mutants expressing the Arabidopsis thaliana enzyme or the homologue from Oryza sativa exhibit resistance toward toxic fluorouridine, fluorouracil, and fluoroorotic acid. Mutants with increased and decreased nucleosidase activity are delayed in germination Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.2.2 additional information
-
 additional information kinetics Arabidopsis thaliana
3.2.2.2 1.4
-
 Inosine recombinant enzyme expressed in Escherichia coli Arabidopsis thaliana
3.2.2.3 additional information
-
 additional information kinetics Arabidopsis thaliana
3.2.2.3 0.8
-
 uridine recombinant enzyme expressed in Escherichia coli Arabidopsis thaliana
3.2.2.3 2.1
-
 uridine recombinant enzyme expressed in Saccharomyces cerevisiae Arabidopsis thaliana
3.2.2.7 additional information
-
 additional information kinetics Arabidopsis thaliana
3.2.2.7 0.7
-
 adenosine recombinant enzyme expressed in Escherichia coli Arabidopsis thaliana
3.2.2.8 additional information
-
 additional information kinetics Arabidopsis thaliana
3.2.2.8 0.4
-
 isopentenyladenine-riboside recombinant enzyme expressed in Escherichia coli, in 50 mM Tris-HCl, pH 7.5, at 30°C Arabidopsis thaliana
3.2.2.8 0.7
-
 adenosine recombinant enzyme expressed in Escherichia coli, in 50 mM Tris-HCl, pH 7.5, at 30°C Arabidopsis thaliana
3.2.2.8 0.8
-
 uridine recombinant enzyme expressed in Escherichia coli, in 50 mM Tris-HCl, pH 7.5, at 30°C Arabidopsis thaliana
3.2.2.8 1.4
-
 Inosine recombinant enzyme expressed in Escherichia coli Arabidopsis thaliana
3.2.2.8 1.4
-
 Inosine recombinant enzyme expressed in Escherichia coli, in 50 mM Tris-HCl, pH 7.5, at 30°C Arabidopsis thaliana
3.2.2.8 2.1
-
 uridine recombinant enzyme expressed in Saccharomyces cerevisiae, in 50 mM Tris-HCl, pH 7.5, at 30°C Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.2 Arabidopsis thaliana
-
-
-
3.2.2.3 Arabidopsis thaliana Q9SJM7
-
-
3.2.2.3 Oryza sativa
-
-
-
3.2.2.7 Arabidopsis thaliana
-
-
-
3.2.2.8 Arabidopsis thaliana
-
-
-
3.2.2.8 Arabidopsis thaliana Q9SJM7 ecotype Columbia
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.8 Ni-NTA column chromatography Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.2.2 guard cell
-
 Arabidopsis thaliana
-
3.2.2.2 pollen
-
 Arabidopsis thaliana
-
3.2.2.2 root
-
 Arabidopsis thaliana
-
3.2.2.2 root tip
-
 Arabidopsis thaliana
-
3.2.2.2 vascular cell of roots and in root tips Arabidopsis thaliana
-
3.2.2.3 guard cell
-
 Arabidopsis thaliana
-
3.2.2.3 pollen
-
 Arabidopsis thaliana
-
3.2.2.3 root
-
 Arabidopsis thaliana
-
3.2.2.3 root tip
-
 Arabidopsis thaliana
-
3.2.2.3 vascular cell of roots and in root tips Arabidopsis thaliana
-
3.2.2.7 guard cell
-
 Arabidopsis thaliana
-
3.2.2.7 pollen
-
 Arabidopsis thaliana
-
3.2.2.7 root
-
 Arabidopsis thaliana
-
3.2.2.7 root tip
-
 Arabidopsis thaliana
-
3.2.2.7 vascular cell of roots and in root tips Arabidopsis thaliana
-
3.2.2.8 guard cell
-
 Arabidopsis thaliana
-
3.2.2.8 pollen
-
 Arabidopsis thaliana
-
3.2.2.8 root
-
 Arabidopsis thaliana
-
3.2.2.8 root tip
-
 Arabidopsis thaliana
-
3.2.2.8 root vascular cell URH1 is mainly transcribed in the vascular cells of roots Arabidopsis thaliana
-
3.2.2.8 vascular cell of roots and in root tips Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.2.2 14.5
-
 purified recombinant enzyme, substrate inosine Arabidopsis thaliana
3.2.2.3 303.3
-
 purified recombinant enzyme, substrate uridine Arabidopsis thaliana
3.2.2.8 14.5
-
 purified recombinant enzyme, substrate inosine Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.2 inosine + H2O
-
 Arabidopsis thaliana D-ribose + hypoxanthine
-
 ?
3.2.2.2 additional information the pure recombinant protein exhibits highest hydrolase activity for uridine, followed by inosine and adenosine Arabidopsis thaliana ?
-
 ?
3.2.2.3 additional information the pure recombinant protein exhibits highest hydrolase activity for uridine, followed by inosine and adenosine Arabidopsis thaliana ?
-
 ?
3.2.2.3 uridine + H2O
-
 Arabidopsis thaliana D-ribose + uracil
-
 ?
3.2.2.7 adenosine + H2O
-
 Arabidopsis thaliana D-ribose + adenine
-
 ?
3.2.2.7 additional information the pure recombinant protein exhibits highest hydrolase activity for uridine, followed by inosine and adenosine Arabidopsis thaliana ?
-
 ?
3.2.2.8 adenosine + H2O
-
 Arabidopsis thaliana adenine + D-ribose
-
 ?
3.2.2.8 adenosine + H2O
-
 Arabidopsis thaliana D-ribose + adenine
-
 ?
3.2.2.8 inosine + H2O
-
 Arabidopsis thaliana hypoxanthine + D-ribose
-
 ?
3.2.2.8 inosine + H2O
-
 Arabidopsis thaliana D-ribose + hypoxanthine
-
 ?
3.2.2.8 isopentenyladenine riboside + H2O
-
 Arabidopsis thaliana ?
-
 ?
3.2.2.8 additional information the pure recombinant protein exhibits highest hydrolase activity for uridine, followed by inosine and adenosine Arabidopsis thaliana ?
-
 ?
3.2.2.8 uridine + H2O the pure recombinant protein exhibits highest hydrolase activity for uridine, followed by inosine and adenosine Arabidopsis thaliana uracil + D-ribose
-
 ?
3.2.2.8 uridine + H2O
-
 Arabidopsis thaliana D-ribose + uracil
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.2.3 URH1
-
 Oryza sativa
3.2.2.3 URH1
-
 Arabidopsis thaliana
3.2.2.3 uridine-ribohydrolase 1
-
 Oryza sativa
3.2.2.3 uridine-ribohydrolase 1
-
 Arabidopsis thaliana
3.2.2.8 URH1 URH1 is a member of the inosine-uridine nucleosidase protein family Arabidopsis thaliana
3.2.2.8 uridine-ribohydrolase 1
-
 Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
3.2.2.3 physiological function uridine-ribohydrolase is a key regulator in the uridine degradation pathway of Arabidopsis thaliana, with a pivotal function of URH1 as regulative in pyrimidine degradation. The enzyme activity must be well balanced in the early phase of plant development Arabidopsis thaliana