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Literature summary extracted from

  • Asega, A.F.; do Nascimento, J.R.; Schroeven, L.; Van den Ende, W.; Carvalho, M.A.
    Cloning, characterization and functional analysis of a 1-FEH cDNA from Vernonia herbacea (Vell.) Rusby (2008), Plant Cell Physiol., 49, 1185-1195.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.153 heterologous expression in Pichia pastoris strain X-33 after culturing of plasmids in Escherichia coli, expression vector pPICZalphaA Vernonia herbacea

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.153 sucrose range 0.5-10 mM, 1 mM inhibits 1-FEH by 8%, 5 mM by about 50%, 10 mM by 81% Vernonia herbacea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.153 65700
-
 calculated from sequence Vernonia herbacea

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.153 Vernonia herbacea A9JIF3 fragment, full-length cDNA of FEH gene of Vernonia herbacea, 2,074 bp, 582 amino acids; samples of sprouting induced plants taken at day 0 and 20, and of defoliated plants stored for one week at 5 degrees Celsius
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.153 concentration by centrifugation after 4 d methanol induction Vernonia herbacea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.153 rhizophore transcripts detected in proximal region of sprouting induced plant, in proximal, medium, and distal regions of sprouting induced + 5°C plants Vernonia herbacea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.153 additional information
-
 activity increase gradually from 1% up to 8% inulin, however solubility above 5% incomplete Vernonia herbacea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.153 1-kestose + H2O from Vernonia herbacea and Vernonia discolor, but levans from Gomphrena macrocephala and Phleum pratense are poorly hydrolyzed, hydrolytic cleavage of terminal fructosyl residues off inulin, sucrose is not hydrolyzed Vernonia herbacea D-fructose + sucrose
-
 ?
3.2.1.153 inulin + H2O 5% inulin from Vernonia herbacea and Vernonia discolor, but levans from Gomphrena macrocephala and Phleum pratense are poorly hydrolyzed, hydrolytic cleavage of terminal fructosyl residues off inulin, sucrose is not hydrolyzed Vernonia herbacea D-fructose + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.153 1-FEH
-
 Vernonia herbacea
3.2.1.153 fructan 1-exohydrolase
-
 Vernonia herbacea

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.153 40
-
-
 Vernonia herbacea

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.153 20 50 at pH 5.5, maximal activity of slightly more than 400 microgram fructose per mg protein and h at 40°C, about 95% of maximal activity at 50°C, about 90% of maximal activity at 30°C, about 50% at 20°C, about 25% of maximal activity at 20°C Vernonia herbacea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.153 5.5
-
 consistent with a vacuolar or extracellular localization Vernonia herbacea

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.153 4 6.5 0.05M citrate phosphate buffer, 30°C, at pH 5.5 maximal activity of more than 300 microgram fructose per mg protein and hour, at pH 4.5-5.0 about 90% activity, at pH 4.25 and 6.0 about 75% activity, at pH 4.0 and 6.5 about 60% activity Vernonia herbacea

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.153 Vernonia herbacea estimation by identification and characterization
-
 5.32