EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.17.4 | into the vector pGEM-T Easy and subsequently into pProEX-1 for expression of the protein in Escherichia coli BL21 cells | Malus domestica |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.17.4 | 0.08939 | - |
1-aminocyclopropane-1-carboxylic acid | - |
Malus domestica | |
1.14.17.4 | 0.2445 | - |
1-aminocyclopropane-1-carboxylic acid | - |
Malus domestica | |
1.14.17.4 | 0.401 | - |
1-aminocyclopropane-1-carboxylic acid | - |
Malus domestica |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.14.17.4 | Fe2+ | - |
Malus domestica |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.17.4 | 35350 | - |
determined by SDA-PAGE and Western blot analysis | Malus domestica |
1.14.17.4 | 36310 | - |
determined by SDA-PAGE and Western blot analysis | Malus domestica |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.17.4 | 1-aminocyclopropane-1-carboxylic acid + ascorbate + O2 | Malus domestica | - |
ethylene + cyanide + dehydroascorbate + CO2 + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.17.4 | Malus domestica | - |
- |
- |
1.14.17.4 | Malus domestica | O48882 | - |
- |
1.14.17.4 | Malus domestica | Q00985 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.17.4 | recombinant ACO is purified by affinity chromatography using nickel nitrilotriacetic acid resin, furthermore Sephadex G-25 resin and a Mono-Q column are used, in addition ACO is extracted from apple leaf and fruit tissue | Malus domestica |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.14.17.4 | fruit | - |
Malus domestica | - |
1.14.17.4 | leaf | - |
Malus domestica | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.17.4 | 1-aminocyclopropane-1-carboxylic acid + ascorbate + O2 | - |
Malus domestica | ethylene + cyanide + dehydroascorbate + CO2 + H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.17.4 | 1-aminocyclopropane-1-carboxylic acid oxidase | - |
Malus domestica |
1.14.17.4 | ACC oxidase | - |
Malus domestica |
1.14.17.4 | ACO | - |
Malus domestica |
1.14.17.4 | MD-ACO1 | - |
Malus domestica |
1.14.17.4 | MD-ACO2 | - |
Malus domestica |
1.14.17.4 | MD-ACO3 | - |
Malus domestica |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.17.4 | 0.0344 | - |
1-aminocyclopropane-1-carboxylic acid | - |
Malus domestica | |
1.14.17.4 | 0.066 | - |
1-aminocyclopropane-1-carboxylic acid | - |
Malus domestica | |
1.14.17.4 | 0.0914 | - |
1-aminocyclopropane-1-carboxylic acid | - |
Malus domestica |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.17.4 | 7.2 | - |
- |
Malus domestica |
1.14.17.4 | 7.5 | - |
- |
Malus domestica |
1.14.17.4 | 7.8 | - |
- |
Malus domestica |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.14.17.4 | 6.5 | 8.5 | - |
Malus domestica |
1.14.17.4 | 6.5 | 8 | - |
Malus domestica |