Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Fialho, L.d.S.; Guimaraes, V.M.; Callegari, C.M.; Reis, A.P.; Barbosa, D.S.; Borges, E.E.; Moreira, M.A.; de Rezende, S.T.
    Characterization and biotechnological application of an acid alpha-galactosidase from Tachigali multijuga Benth. seeds (2008), Phytochemistry, 69, 2579-2585.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.22 Ag+
-
 Tachigali multijuga
3.2.1.22 Cu2+
-
 Tachigali multijuga
3.2.1.22 D-galactose competitive Tachigali multijuga
3.2.1.22 Hg2+
-
 Tachigali multijuga
3.2.1.22 melibiose
-
 Tachigali multijuga
3.2.1.22 Sodium dodecyl sulfate
-
 Tachigali multijuga

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.22 0.45
-
 4-nitrophenyl alpha-D-galactoside pH 5.0, 40°C Tachigali multijuga
3.2.1.22 5.37
-
 melibiose pH 5.0, 40°C Tachigali multijuga
3.2.1.22 39.62
-
 raffinose pH 5.0, 40°C Tachigali multijuga
3.2.1.22 48.8
-
 stachyose pH 5.0, 40°C Tachigali multijuga

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.22 34000
-
 gel filtration Tachigali multijuga
3.2.1.22 38000
-
 1 * 38000 Tachigali multijuga

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.22 Tachigali multijuga
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.22 from germination seed Tachigali multijuga

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.22 seed activity increases during seed germination and reaches a maximum level at 108 h after seed imbibition Tachigali multijuga
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.22 83.6
-
 pH 5.0, 40°C Tachigali multijuga

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.22 4-nitrophenyl alpha-D-galactoside + H2O
-
 Tachigali multijuga 4-nitrophenol + alpha-D-galactose
-
 ?
3.2.1.22 galactomannan + H2O
-
 Tachigali multijuga ?
-
 ?
3.2.1.22 melibiose + H2O
-
 Tachigali multijuga D-galactose + D-glucose
-
 ?
3.2.1.22 raffinose + H2O
-
 Tachigali multijuga sucrose + D-galactose
-
 ?
3.2.1.22 stachyose + H2O
-
 Tachigali multijuga ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.22 monomer 1 * 38000 Tachigali multijuga

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.22 50
-
-
 Tachigali multijuga

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.22 40
-
 stable Tachigali multijuga
3.2.1.22 50
-
 30 min, 79% loss of activity Tachigali multijuga

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.22 5 5.5
-
 Tachigali multijuga

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.22 7
-
 complete loss of activity Tachigali multijuga

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.22 2.74
-
 D-galactose pH 5.0, 40°C Tachigali multijuga