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Literature summary extracted from

  • Xu, D.; Shen, Y.; Chappell, J.; Cui, M.; Nielsen, M.T.
    Biochemical and molecular characterizations of nicotine demethylase in tobacco (2007), Physiol. Plant., 129, 307-319.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.B11 D121-AA8 cDNA transformed into yeast cells Nicotiana tabacum
1.14.14.B11 expression in yeast Nicotiana tabacum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.14.B11 1-aminobenzotriazole at 1 mM inhibits by 17% Nicotiana tabacum
1.14.14.B11 Chloropromazine 1 mM, 87% inhibition of recombinant nicotine demethylase activity in yeast microsomes Nicotiana tabacum
1.14.14.B11 chlorpromazine at 1 mM inhibits by 87% Nicotiana tabacum
1.14.14.B11 CO partial reverse activity after light exposure Nicotiana tabacum
1.14.14.B11 cytochrome c 1 mM, 78% inhibition of recombinant nicotine demethylase activity in yeast microsomes Nicotiana tabacum
1.14.14.B11 oxidized cytochrome c at 0.1 mM inhibits by 78% Nicotiana tabacum
1.14.14.B11 Piperonylbutoxide at 1 mM inhibits by 42% Nicotiana tabacum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.B11 0.0039
-
 Nicotine
-
 Nicotiana tabacum
1.14.14.B11 0.0039
-
 Nicotine at 25°C, pH 6.25 Nicotiana tabacum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.14.B11 membrane putative N-terminal hydrophobic trans-membrane domain Nicotiana tabacum 16020
-
1.14.14.B11 microsome
-
 Nicotiana tabacum
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.14.B11 Iron cysteine-containing heme-binding region Nicotiana tabacum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.B11 nicotine + NADPH + O2 + H+ Nicotiana tabacum demethylates nicotine to form nornicotine, a precursor to the nitrosamine N'-nitrosonornicotine nornicotine + formaldehyde + NADP+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.B11 Nicotiana tabacum Q38Q87
-
-
1.14.14.B11 Nicotiana tabacum Q38Q87 var. 4407
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.B11 leaf
-
 Nicotiana tabacum
-
1.14.14.B11 leaf induced in leaves treated with the growth regulator ethylene Nicotiana tabacum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.B11 nicotine + NADPH + O2 + H+
-
 Nicotiana tabacum nornicotine + formaldehyde + NADP+ + H2O
-
 ?
1.14.14.B11 nicotine + NADPH + O2 + H+ demethylates nicotine to form nornicotine, a precursor to the nitrosamine N'-nitrosonornicotine Nicotiana tabacum nornicotine + formaldehyde + NADP+ + H2O
-
 ?
1.14.14.B11 nicotine + NADPH + O2 + H+ six putative substrate recognition sites Nicotiana tabacum nornicotine + formaldehyde + NADP+ + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.B11 CYP82E4
-
 Nicotiana tabacum

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.B11 cytochrome P450
-
 Nicotiana tabacum
1.14.14.B11 NADPH
-
 Nicotiana tabacum
1.14.14.B11 NADPH is required for full activity Nicotiana tabacum

Expression

EC Number Organism Comment Expression
1.14.14.B11 Nicotiana tabacum ethylene induces microsomal demethylase activity in leaves up

General Information

EC Number General Information Comment Organism
1.14.14.B11 physiological function only microsomes from yeast expressing the D121-AA8 cDNA exhibit significant nicotine demethylase activity Nicotiana tabacum