Literature summary extracted from
Liu, Z.; Lu, D.; Li, J.; Chen, W.; Liu, Z.
Strengthening intersubunit hydrogen bonds for enhanced stability of recombinant urate oxidase from Aspergillus flavus: molecular simulations and experimental validation (2009), Phys. Chem. Chem. Phys., 11, 333-340.
Application
EC Number |
Application |
Comment |
Organism |
---|
1.7.3.3 |
medicine |
substitute for allopurinol in the management of gout and hyperuricaemia |
Aspergillus flavus |
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
1.7.3.3 |
expressed in Escherichia coli BL21 (DE3) |
Aspergillus flavus |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
1.7.3.3 |
urate + O2 + H2O |
Aspergillus flavus |
first step in uric acid degradation |
5-hydroxyisourate + H2O2 |
- |
? |
|
Organic Solvent Stability
EC Number |
Organic Solvent |
Comment |
Organism |
---|
1.7.3.3 |
DMSO |
the residual recombinant UOX activity in the presence of DMSO is significantly higher than that in pure H2O, the residual UOX activity increases in response to the increase in the DMSO concentration up to 20%, further increase in DMSO concentration (50-70%) results in significant UOX deactivation |
Aspergillus flavus |
1.7.3.3 |
Methanol |
the residual recombinant UOX activity in the presence of methanol is significantly higher than that in pure H2O, the residual UOX activity increases in response to the increase in the methanol concentration up to 20%, further increase in methanol concentration (50-70%) results in significant UOX deactivation |
Aspergillus flavus |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.7.3.3 |
Aspergillus flavus |
Q00511 |
- |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.7.3.3 |
urate + O2 + H2O |
first step in uric acid degradation |
Aspergillus flavus |
5-hydroxyisourate + H2O2 |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
1.7.3.3 |
homotetramer |
determined by gel filtration, tunnel-shaped homotetramer, each of the 4 active sites in UOX is formed by residues from 2 subunits and located at the subunit-subunit interface |
Aspergillus flavus |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
1.7.3.3 |
Rasburicase |
commercially available recombinant enzyme |
Aspergillus flavus |
1.7.3.3 |
Uox |
- |
Aspergillus flavus |
1.7.3.3 |
Urate oxidase |
- |
Aspergillus flavus |
Temperature Stability [°C]
EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Comment |
Organism |
---|
1.7.3.3 |
additional information |
- |
thermal deactivation of recombinant UOX at neutral pH is associated with the loss of intersubunit hydrogen bonds, subunit is unstable at room temperature and unfolds rapidly at 100°C, tetramer has significantly higher stability than its subunit |
Aspergillus flavus |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
1.7.3.3 |
8.9 |
- |
assay at |
Aspergillus flavus |
pH Stability
EC Number |
pH Stability |
pH Stability Maximum |
Comment |
Organism |
---|
1.7.3.3 |
7 |
- |
UOX is deactivated at different protein concentrations at 45°C in 20 mM phosphate containing 0.15 M NaCl, 0.01 mg/ml UOX is deactivated much faster than its counterparts at concentrations of 0.1 and 1.0 mg/ml |
Aspergillus flavus |