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Literature summary extracted from
- The effective role of positive charge saturation in bioluminescence color and thermostability of firefly luciferase (2009), Photochem. Photobiol. Sci., 8, 847-855.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.13.12.7 | expression in Escherichia coli BL21 | Lampyris turkestanicus |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.13.12.7 | E353Q/ins356R | emission of red and green light at pH 7.8, 20% less activity | Lampyris turkestanicus |
| 1.13.12.7 | E354K | emission of red and green light at pH 7.8, 26% higher activity | Lampyris turkestanicus |
| 1.13.12.7 | E354K/ins356R | emission of red and green light at pH 7.8, 20% higher activity | Lampyris turkestanicus |
| 1.13.12.7 | E354R | emission of red and green light at pH 7.8, 33% higher activity | Lampyris turkestanicus |
| 1.13.12.7 | E354R/ins356R | emission of red light at pH 7.8 compared to green emitted light by wild-type luciferase, 53% higher activity compared to wild-type luciferase | Lampyris turkestanicus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.13.12.7 | 0.011 | - |
D-luciferin | mutant E354K/ins356K | Lampyris turkestanicus |  |
| 1.13.12.7 | 0.012 | - |
D-luciferin | mutant E354Q/ins356K | Lampyris turkestanicus | |
| 1.13.12.7 | 0.013 | - |
D-luciferin | mutant E354K | Lampyris turkestanicus | |
| 1.13.12.7 | 0.014 | - |
D-luciferin | mutant E354R/ins356K | Lampyris turkestanicus | |
| 1.13.12.7 | 0.016 | - |
D-luciferin | mutant E354R | Lampyris turkestanicus | |
| 1.13.12.7 | 0.023 | - |
D-luciferin | wild-type luciferase | Lampyris turkestanicus | |
| 1.13.12.7 | 0.04 | - |
ATP | mutant E354K/ins356K | Lampyris turkestanicus | |
| 1.13.12.7 | 0.05 | - |
ATP | mutant E354Q/ins356K | Lampyris turkestanicus | |
| 1.13.12.7 | 0.063 | - |
ATP | mutant E354K | Lampyris turkestanicus | |
| 1.13.12.7 | 0.071 | - |
ATP | wild-type luciferase | Lampyris turkestanicus | |
| 1.13.12.7 | 0.095 | - |
ATP | mutant E354R/ins356K | Lampyris turkestanicus | |
| 1.13.12.7 | 0.115 | - |
ATP | mutant E354R | Lampyris turkestanicus | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.13.12.7 | Lampyris turkestanicus | Q5UFR2 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.13.12.7 | Ni-NTA sepharose, 95% purity | Lampyris turkestanicus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.13.12.7 | additional information | - |
12000000000 relative light units/s/mg, mutant E354Q/ins356K | Lampyris turkestanicus |
| 1.13.12.7 | additional information | - |
15000000000 relative light units/s/mg, wild-type luciferase | Lampyris turkestanicus |
| 1.13.12.7 | additional information | - |
18000000000 relative light units/s/mg, mutant E354K/ins356K | Lampyris turkestanicus |
| 1.13.12.7 | additional information | - |
19000000000 relative light units/s/mg, mutant E354K | Lampyris turkestanicus |
| 1.13.12.7 | additional information | - |
20000000000 relative light units/s/mg, mutant E354R | Lampyris turkestanicus |
| 1.13.12.7 | additional information | - |
23000000000 relative light units/s/mg, mutnat E354R/ins356K | Lampyris turkestanicus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.13.12.7 | D-luciferin + ATP + O2 | - |
Lampyris turkestanicus | oxidized luciferin + AMP + CO2 + diphosphate + hv | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.13.12.7 | firefly luciferase | - |
Lampyris turkestanicus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.13.12.7 | 25 | - |
assay at | Lampyris turkestanicus |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.13.12.7 | 25 | 35 | depending on mutation | Lampyris turkestanicus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.13.12.7 | 32 | - |
mutant E354R, E354K, E354R/ins356Arg with greater thermostability than wild-type luciferase | Lampyris turkestanicus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.13.12.7 | 7.8 | - |
assay at | Lampyris turkestanicus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.13.12.7 | 7.5 | 8.5 | depending on mutation | Lampyris turkestanicus |
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Release 2023.1 (January 2023)
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