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Literature summary extracted from

  • Suzuki, Y.; Soga, K.; Yoshimatsu, K.; Shioi, Y.
    Expression and purification of pheophorbidase, an enzyme catalyzing the formation of pyropheophorbide during chlorophyll degradation: comparison with the native enzyme (2008), Photochem. Photobiol. Sci., 7, 1260-1266.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.82 recombinant PPD is produced using a glutathione S-transferase (GST) fusion system. The PPD and GST genes are fused to a pGEX-2T vector and expressed in Escherichia coli BL21(DE3)pLysS strain under the control of a T7 promoter as a fusion protein Raphanus sativus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.82 0.955
-
 pheophorbide a PPD-GST has about 6fold lower affinity for Pheid a than native PPDs Raphanus sativus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.82 55000
-
 PPD-GST fusion protein, SDS-PAGE Raphanus sativus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.82 pheophorbide a + H2O Raphanus sativus
-
 pyropheophorbide a + methanol + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.82 Raphanus sativus Q2V0W1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.82 single-step affinity chromatography through a GSTrap FF column Raphanus sativus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.82 cotyledon
-
 Raphanus sativus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.82 pheophorbide a + H2O
-
 Raphanus sativus pyropheophorbide a + methanol + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.82 pheophorbidase
-
 Raphanus sativus
3.1.1.82 Ppd
-
 Raphanus sativus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.82 30 80
-
 Raphanus sativus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.82 65
-
 50% loss of activity Raphanus sativus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.82 7 7.5 60 mM MES-HEPES-tricine buffer is used as an incubation medium in the pH range of 5.5 to 9.0 Raphanus sativus