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Literature summary extracted from

  • Awad, R.; Crump, K.; Mullally, M.; Sardana, R.K.; Arnason, J.T.; Trudeau, V.L.
    An improved method for production of recombinant human glutamic acid decarboxylase 65 for use in phytopharmaceutical assessment (2008), Pharm. Biol., 46, 72-81.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.1.15 asiaticoside 20% activation at 0.01 mg/ml Homo sapiens
4.1.1.15 asiaticoside an increase in GAD65 activity by 22% is observed with asiaticoside Homo sapiens
4.1.1.15 asiaticoside an increase in GAD65 activity by 4.5% is observed with asiaticoside Homo sapiens
4.1.1.15 bilobalide 20% activation at 0.01 mg/ml Homo sapiens
4.1.1.15 bilobalide an increase in GAD65 activity by approximately 18% is observed with bilobalide Homo sapiens
4.1.1.15 bilobalide an increase in GAD65 activity by 17.8% is observed with bilobalide Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.15 expressed in Escherichia coli Homo sapiens
4.1.1.15 expressed in Escherichia coli DH5alpha cells Homo sapiens
4.1.1.15 gene gadB, expression in Escherichia coli strain DH5alpha using plasmids pMAL-c2X and the pGEX-3X and fusion to the maltose binding protein or GST, cytoplasmic targeting, overview Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.15 (E)-pent-2-enoic acid 20% inhibition at 0.01 mg/ml Homo sapiens
4.1.1.15 3-Mercaptopropionic acid 43% inhibition at 0.1 mM Homo sapiens
4.1.1.15 allylglycine
-
Homo sapiens
4.1.1.15 asiaticoside
-
Homo sapiens
4.1.1.15 betulinic acid 27% inhibition at 0.01 mg/ml Homo sapiens
4.1.1.15 bilobalide
-
Homo sapiens
4.1.1.15 ginsenosides 23% inhibition at 0.01 mg/ml, ethanol extract of Panax quinquefolius, different glycoside derivatives, overview Homo sapiens
4.1.1.15 additional information aasiaticoside does not inhibit GAD activity even at the highest dose of 0.03 mg/ml. Allylglycine does not inhibit GAD activity even at the highest dose of 0.1 mM; asiaticoside does not inhibit GAD activity even at the highest dose of 0.03 mg/ml, allylglycine exhibits no enzyme inhibition even at the highest dose of 0.1 mM; minor inhibition is seen with the ethanol extract of Panax quinquefolius (23%), betulinic acid (27%), and valerenic acid (20%); minor inhibition is seen with the ethanol extract of Panax quinquefolius (ginsenosides) (23%) Homo sapiens
4.1.1.15 ursolic acid 11.2% inhibition at 0.01 mg/ml Homo sapiens
4.1.1.15 valerenic acid 20% inhibition at 0.01 mg/ml Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.15 65000
-
SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.15 L-glutamate Homo sapiens
-
4-aminobutanoate + CO2
-
?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.15 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.15 amylose resin column chromatography, glutathione Sepharose 4B resin column chromatography Homo sapiens
4.1.1.15 glutathione sepharose 4B resin column chromatography and amylose resin column chromatography Homo sapiens
4.1.1.15 recombinant tagged enzyme from Escherichia coli strain DH5alpha Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.1.15 nervous system two isoforms ofGAD, GAD67and GAD65 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.15 L-glutamate
-
Homo sapiens 4-aminobutanoate + CO2
-
?

Subunits

EC Number Subunits Comment Organism
4.1.1.15 ? x * 65000, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
4.1.1.15 GAD65
-
Homo sapiens
4.1.1.15 GAD65 isoform Homo sapiens
4.1.1.15 glutamic acid decarboxylase 65
-
Homo sapiens
4.1.1.15 glutamic acid decarboxylase 65 isoform Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.15 37
-
assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.15 pyridoxal 5'-phosphate
-
Homo sapiens
4.1.1.15 pyridoxal 5'-phosphate dependent on Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
4.1.1.15 0.0123
-
-
Homo sapiens 3-Mercaptopropionic acid
4.1.1.15 0.0123
-
at 37°C Homo sapiens 3-Mercaptopropionic acid
4.1.1.15 0.0123
-
at 37°C, pH not specified in the publication Homo sapiens 3-Mercaptopropionic acid