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Literature summary extracted from

  • Giovannetti, E.; Laan, A.C.; Vasile, E.; Tibaldi, C.; Nannizzi, S.; Ricciardi, S.; Falcone, A.; Danesi, R.; Peters, G.J.
    Correlation between cytidine deaminase genotype and gemcitabine deamination in blood samples (2008), Nucleosides Nucleotides Nucleic Acids, 27, 720-725.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.4.5 diagnostics evaluation of CDA genotype may be helpful in screening patients before treatment, in order to identify subjects with slower CDA-mediated gemcitabine metabolism Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.5 cytidine + H2O Homo sapiens
-
 uridine + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.5 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.4.5 blood
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.4.5 0.536
-
 activity in non-small cell lung cancer patients with CDA Lys27Lys protein, in 50 mM beta-mercaptoethanol, 0.1 M Tris-HCl, pH 8.0, substrate 0.25 mM gemcitabine Homo sapiens
3.5.4.5 0.7254
-
 activity in non-small cell lung cancer patients carrying the Lys27Gln polymorphism and the Gln27Gln polymorphism, in 50 mM beta-mercaptoethanol, 0.1 M Tris-HCl, pH 8.0, substrate 0.25 mM gemcitabine Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.5 cytidine + H2O
-
 Homo sapiens uridine + NH3
-
 ?
3.5.4.5 gemcitabine + H2O CDA is the major enzyme involved in gemcitabine inactivation, a cytotoxic drug commonly used in the treatment of pancreas and non-small cell lung cancer Homo sapiens 2'-deoxy-2',2'-difluorouridine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.4.5 CDA
-
 Homo sapiens
3.5.4.5 cytidine deaminase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.4.5 37
-
 assay at Homo sapiens