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Literature summary extracted from

  • Koistinen, H.; Koistinen, R.; Zhang, W.M.; Valmu, L.; Stenman, U.H.
    Nexin-1 inhibits the activity of human brain trypsin (2009), Neuroscience, 160, 97-102.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.21.4 enterokinase trypsinogens (0.0002 mM) are incubated at room temperature with 6-200 ng enterokinase in 0.1 M Tris (pH 8), containing 5 mM CaCl2 and 0.01% bovine serum albumin for 10-60 min Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.4 inactive trypsinogen-4 is expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.4 Aprotinin very efficient inhibition of trypsin-1 and trypsin-2, but not of trypsin-4 Homo sapiens
3.4.21.4 nexin-1 nexin-1 inhibits isozymes trypsin-1 ad trypsin-2, nexin-1 inhibits trypsin-4 and forms stable complexes only with this trypsin isoenzyme Homo sapiens
3.4.21.4 Pefabloc SC completely inhibits isoenzymes trypsin-1, trypsin-2, and trypsin-4 Homo sapiens
3.4.21.4 tumor-associated trypsin inhibitor very efficient inhibition of trypsin-1 and trypsin-2, but not of trypsin-4 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.4 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.4 Ni-NTA Sepharose column chromatography and Resource S column chromatography Homo sapiens

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.4.21.4 denatured trypsinogen is rapidly diluted with re-folding buffer (0.7 M guanidine-HCl, 0.1 M Tris-HCl, pH 8.0, containing 10 mM benzamidine, 2 mM L-cysteine, and 1 mM L-cystine) Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.4 brain trypsinogen-4 is the only trypsin-isoenzyme expressed in the brain Homo sapiens
-
3.4.21.4 pancreas isozymes trypsin-1 and trypsin-2 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.4 N-benzoyl-L-isoleucyl-L-glutamyl-glycyl-L-arginine methyl ester + H2O
-
 Homo sapiens N-benzoyl-L-isoleucyl-L-glutamyl-glycyl-L-arginine + methanol
-
 ?
3.4.21.4 N-benzoyl-L-isoleucyl-L-glutamyl-glycyl-L-arginine-4-nitroanilide + H2O
-
 Homo sapiens N-benzoyl-L-isoleucyl-L-glutamyl-glycyl-L-arginine + 4-nitroaniline
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.4 trypsin-1 isozyme Homo sapiens
3.4.21.4 trypsin-2 isozyme Homo sapiens
3.4.21.4 trypsin-4 brain isozyme Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.4 23.5
-
 N-benzoyl-L-isoleucyl-L-glutamyl-glycyl-L-arginine-4-nitroanilide isozyme trypsin-1, in 50 mM Tris, pH 8.0, 100 mM NaCl, 6 mM CaCl2 Homo sapiens
3.4.21.4 32.7
-
 N-benzoyl-L-isoleucyl-L-glutamyl-glycyl-L-arginine-4-nitroanilide isozyme trypsin-2, in 50 mM Tris, pH 8.0, 100 mM NaCl, 6 mM CaCl2 Homo sapiens
3.4.21.4 70.5
-
 N-benzoyl-L-isoleucyl-L-glutamyl-glycyl-L-arginine-4-nitroanilide isozyme trypsin-4, in 50 mM Tris, pH 8.0, 100 mM NaCl, 6 mM CaCl2 Homo sapiens