EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.4.21.68 | dibutyryl-cAMP | slightly increases level of tPA mRNA expression. Concentration-dependently increases tPA activity in both basal and lipopolysaccharide-stimulated rat primary astrocytes. Treatment with 100 micromol increases tPA activity by 757.9% compared with lipopolysaccharide treatment. Differentially modulates MMP-9 and tPA activity through a mechanism related to PKA activation. Co-treatment with lipopolysaccharide and dibutyryl-cAMP does not have an additive effect on tPA mRNA induction | Rattus norvegicus | |
3.4.21.68 | IBMX | increases tPA activity in lipopolysaccharide-stimulated rat primary astrocytes | Rattus norvegicus | |
3.4.21.68 | lipopolysaccharide | slightly increases level of tPA mRNA expression. Co-treatment with lipopolysaccharide and dibutyryl-cAMP does not have an additive effect on tPA mRNA induction | Rattus norvegicus | |
3.4.21.68 | rolipram | increases tPA activity in lipopolysaccharide-stimulated rat primary astrocytes | Rattus norvegicus |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.4.21.68 | astrocytes co-transfected with metalloproteinase-9 or tPA luciferase reporter plasmid and pCMV-beta-galactosidase reporter plasmid | Rattus norvegicus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.21.68 | H89 | inhibits dibutyryl-cAMP-mediated changes in tPA activity without affecting metalloproteinase-9 activity | Rattus norvegicus | |
3.4.21.68 | lipopolysaccharide | decreases tPA activity. Co-treatment with dibutyryl-cAMP and lipopolysaccharide dose-dependently prevents lipopolysaccharide-induced downregulation of tPA activity | Rattus norvegicus | |
3.4.21.68 | plasminogen activator inhibitor-1 | plays a major role in the regulation of tPA activity | Rattus norvegicus | |
3.4.21.68 | Rp-cAMP | inhibits dibutyryl-cAMP-mediated changes in tPA activity without affecting metalloproteinase-9 activity | Rattus norvegicus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.21.68 | 68000 | - |
casein zymography or gel filtration | Rattus norvegicus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.21.68 | Rattus norvegicus | - |
Sprague-Dawley rats | - |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.4.21.68 | additional information | primary astrocyte | Rattus norvegicus | - |
3.4.21.68 | pup | 1- to 2-day-old pups | Rattus norvegicus | - |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.21.68 | Tissue plasminogen activator | - |
Rattus norvegicus |
3.4.21.68 | tPA | - |
Rattus norvegicus |