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Literature summary extracted from

  • Santos, E.B.; Yeh, R.; Lee, J.; Nikhamin, Y.; Punzalan, B.; Punzalan, B.; La Perle, K.; Larson, S.M.; Sadelain, M.; Brentjens, R.J.
    Sensitive in vivo imaging of T cells using a membrane-bound Gaussia princeps luciferase (2009), Nat. Med., 15, 338-344.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.13.12.5 additional information membrane-anchored GLuc leads to an 9fold increased bioluminescent signal in fibroblasts compared to GLuc Gaussia princeps

Application

EC Number Application Comment Organism
1.13.12.5 additional information imaging of T cells in the context of a competent immune system, bioluminescent imaging Gaussia princeps

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.12.5 expression in mouse and human C57/BL6 primary T cells, expression in PG-13 cells Renilla sp.
1.13.12.5 expression in mouse and human C57/BL6 primary T cells, expression in PG-13 cells Gaussia princeps

Protein Variants

EC Number Protein Variants Comment Organism
1.13.12.5 extFLuc membrane-anchored Renilla sp.
1.13.12.5 extGLuc membrane-anchored Gaussia princeps

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.13.12.5 cytosol
-
 Renilla sp. 5829
-
1.13.12.5 cytosol
-
 Gaussia princeps 5829
-
1.13.12.5 membrane membrane-anchored Renilla sp. 16020
-
1.13.12.5 membrane membrane-anchored Gaussia princeps 16020
-

Organism

EC Number Organism UniProt Comment Textmining
1.13.12.5 Gaussia princeps
-
-
-
1.13.12.5 Renilla sp.
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.12.5 coelenterazine + O2
-
 Renilla sp. ?
-
 ?
1.13.12.5 coelenterazine + O2
-
 Gaussia princeps ?
-
 ?
1.13.12.5 D-luciferin + O2
-
 Renilla sp. ?
-
 ?
1.13.12.5 D-luciferin + O2
-
 Gaussia princeps ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.12.5 extGLuc
-
 Gaussia princeps
1.13.12.5 extRLuc
-
 Renilla sp.
1.13.12.5 Gaussia luciferase enzyme
-
 Gaussia princeps
1.13.12.5 membrane-anchored RLUc
-
 Renilla sp.
1.13.12.5 Renilla luciferase
-
 Renilla sp.
1.13.12.5 RLuc
-
 Renilla sp.