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Literature summary extracted from
- Cloning of Lentinula edodes lemnp2, a manganese peroxidase that is secreted abundantly in sawdust medium (2009), Mycoscience, 50, 116-122.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 1.11.1.13 | degradation | lingnin-degrading enzymes possess oxidative activity against phenolic compoundss, which can be used for bioremediation, biobleaching, and biofuel production | Lentinula edodes |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.11.1.13 | lemnp2 is cloned by 3'-RACE using cDNA that is synthesized from mycelial RNA | Lentinula edodes |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.11.1.13 | extracellular | secreted into the medium | Lentinula edodes | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.11.1.13 | Manganese | - |
Lentinula edodes |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.11.1.13 | 37000 | - |
theoretical | Lentinula edodes |
| 1.11.1.13 | 44800 | - |
determined by SDS-PAGE | Lentinula edodes |
| 1.11.1.13 | 48000 | - |
determined by gel filtration | Lentinula edodes |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.11.1.13 | Mn2+ + H+ + H2O2 | Lentinula edodes | - |
Mn3+ + H2O | - |
? | |
| 1.11.1.13 | Mn2+ + H+ + H2O2 | Lentinula edodes SR-1 | - |
Mn3+ + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.11.1.13 | Lentinula edodes | B5U990 | - |
- |
| 1.11.1.13 | Lentinula edodes SR-1 | B5U990 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.11.1.13 | using a Toyopearl Butyl-650M, a Toyopearl DEAE-650 and a Superdex 75 HR 10/30 column | Lentinula edodes |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.11.1.13 | Mn2+ + guaiacol + H2O2 | activity assay | Lentinula edodes | ? | - |
? | |
| 1.11.1.13 | Mn2+ + guaiacol + H2O2 | activity assay | Lentinula edodes SR-1 | ? | - |
? | |
| 1.11.1.13 | Mn2+ + H+ + H2O2 | - |
Lentinula edodes | Mn3+ + H2O | - |
? | |
| 1.11.1.13 | Mn2+ + H+ + H2O2 | - |
Lentinula edodes SR-1 | Mn3+ + H2O | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.11.1.13 | monomer | - |
Lentinula edodes |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.11.1.13 | LeMnP2 | - |
Lentinula edodes |
| 1.11.1.13 | manganese peroxidase | - |
Lentinula edodes |
| 1.11.1.13 | MnP | - |
Lentinula edodes |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.11.1.13 | 30 | - |
activity assay | Lentinula edodes |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.11.1.13 | 4 | - |
activity assay | Lentinula edodes |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 1.11.1.13 | Lentinula edodes | theoretical | - |
3.98 |
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