BRENDA - Enzyme Database

In vitro characterization of a carotenoid cleavage dioxygenase from Nostoc sp. PCC 7120 reveals a novel cleavage pattern, cytosolic localization and induction by highlight

Scherzinger, D.; Al-Babili, S.; Mol. Microbiol. 69, 231-244 (2008) View publication on PubMed

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.13.11.51
additional information
induction of enzyme activity with highlight treatment
Nostoc sp. PCC 7120 = FACHB-418
Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
1.13.11.51
expression as a glutathion-S-transferase fusion protein in Escherichia coli BL21
Nostoc sp. PCC 7120 = FACHB-418
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.13.11.51
additional information
decreased enzyme activtiy at lower temperature
Nostoc sp. PCC 7120 = FACHB-418
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.13.11.51
cytosol
-
Nostoc sp. PCC 7120 = FACHB-418
5829
-
1.13.11.51
soluble
-
Nostoc sp. PCC 7120 = FACHB-418
-
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.13.11.51
55000
-
value about, Western blot
Nostoc sp. PCC 7120 = FACHB-418
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.13.11.51
Nostoc sp. PCC 7120 = FACHB-418
Q8YXV3
Anabaena sp. PCC 7120
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
1.13.11.51
-
Nostoc sp. PCC 7120 = FACHB-418
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.13.11.51
3-OH-beta-apo-10'-carotenal + O2
assay at pH 7.8, 28°C, incubation stopped by adding one volume of acetone
700272
Nostoc sp. PCC 7120 = FACHB-418
apo-10',10-apocarotene-dial + ?
-
-
-
?
1.13.11.51
3-OH-beta-apo-8'-carotenal + O2
assay at pH 7.8, 28°C, incubation stopped by adding one volume of acetone
700272
Nostoc sp. PCC 7120 = FACHB-418
apo-8',10-apocarotene-dial
-
-
-
?
1.13.11.51
4-hydroxymyxol 2'-fucoside + O2
assay at pH 7.8, 28°C, incubation stopped by adding one volume of acetone
700272
Nostoc sp. PCC 7120 = FACHB-418
?
-
-
-
?
1.13.11.51
4-ketomyxol 2'-fucoside + O2
assay at pH 7.8, 28°C, incubation stopped by adding one volume of acetone
700272
Nostoc sp. PCC 7120 = FACHB-418
?
-
-
-
?
1.13.11.51
astaxanthin + O2
assay at pH 7.8, 28°C, incubation stopped by adding one volume of acetone
700272
Nostoc sp. PCC 7120 = FACHB-418
?
-
-
-
?
1.13.11.51
beta-apo-10'-carotenal + O2
assay at pH 7.8, 28°C, incubation stopped by adding one volume of acetone
700272
Nostoc sp. PCC 7120 = FACHB-418
?
-
-
-
?
1.13.11.51
beta-apo-8'-carotenal + O2
assay at pH 7.8, 28°C, incubation stopped by adding one volume of acetone
700272
Nostoc sp. PCC 7120 = FACHB-418
?
-
-
-
?
1.13.11.51
canthaxanthin + O2
assay at pH 7.8, 28°C, incubation stopped by adding one volume of acetone
700272
Nostoc sp. PCC 7120 = FACHB-418
?
-
-
-
?
1.13.11.51
echinenone + O2
assay at pH 7.8, 28°C, incubation stopped by adding one volume of acetone
700272
Nostoc sp. PCC 7120 = FACHB-418
?
-
-
-
?
1.13.11.51
gamma-carotene + O2
assay at pH 7.8, 28°C, incubation stopped by adding one volume of acetone
700272
Nostoc sp. PCC 7120 = FACHB-418
?
-
-
-
?
1.13.11.51
myxol + O2
assay at pH 7.8, 28°C, incubation stopped by adding one volume of acetone
700272
Nostoc sp. PCC 7120 = FACHB-418
?
-
-
-
?
1.13.11.51
myxol 2'-fucoside + O2
assay at pH 7.8, 28°C, incubation stopped by adding one volume of acetone
700272
Nostoc sp. PCC 7120 = FACHB-418
?
-
-
-
?
1.13.11.51
zeaxanthin + O2
assay at pH 7.8, 28°C, incubation stopped by adding one volume of acetone
700272
Nostoc sp. PCC 7120 = FACHB-418
?
-
-
-
?
Synonyms
EC Number
Synonyms
Commentary
Organism
1.13.11.51
carotenoid cleavage dioxygenase
-
Nostoc sp. PCC 7120 = FACHB-418
1.13.11.51
NosCCD
-
Nostoc sp. PCC 7120 = FACHB-418
1.13.11.51
Nostoc carotenoid cleavage dioxygenase
-
Nostoc sp. PCC 7120 = FACHB-418
1.13.11.51
NSC1
-
Nostoc sp. PCC 7120 = FACHB-418
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.13.11.51
28
-
at 20°C decreased enzyme acitivity
Nostoc sp. PCC 7120 = FACHB-418
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.13.11.51
7.8
-
assay at
Nostoc sp. PCC 7120 = FACHB-418
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.13.11.51
additional information
induction of enzyme activity with highlight treatment
Nostoc sp. PCC 7120 = FACHB-418
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.13.11.51
expression as a glutathion-S-transferase fusion protein in Escherichia coli BL21
Nostoc sp. PCC 7120 = FACHB-418
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.13.11.51
additional information
decreased enzyme activtiy at lower temperature
Nostoc sp. PCC 7120 = FACHB-418
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.13.11.51
cytosol
-
Nostoc sp. PCC 7120 = FACHB-418
5829
-
1.13.11.51
soluble
-
Nostoc sp. PCC 7120 = FACHB-418
-
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.13.11.51
55000
-
value about, Western blot
Nostoc sp. PCC 7120 = FACHB-418
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.13.11.51
-
Nostoc sp. PCC 7120 = FACHB-418
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.13.11.51
3-OH-beta-apo-10'-carotenal + O2
assay at pH 7.8, 28°C, incubation stopped by adding one volume of acetone
700272
Nostoc sp. PCC 7120 = FACHB-418
apo-10',10-apocarotene-dial + ?
-
-
-
?
1.13.11.51
3-OH-beta-apo-8'-carotenal + O2
assay at pH 7.8, 28°C, incubation stopped by adding one volume of acetone
700272
Nostoc sp. PCC 7120 = FACHB-418
apo-8',10-apocarotene-dial
-
-
-
?
1.13.11.51
4-hydroxymyxol 2'-fucoside + O2
assay at pH 7.8, 28°C, incubation stopped by adding one volume of acetone
700272
Nostoc sp. PCC 7120 = FACHB-418
?
-
-
-
?
1.13.11.51
4-ketomyxol 2'-fucoside + O2
assay at pH 7.8, 28°C, incubation stopped by adding one volume of acetone
700272
Nostoc sp. PCC 7120 = FACHB-418
?
-
-
-
?
1.13.11.51
astaxanthin + O2
assay at pH 7.8, 28°C, incubation stopped by adding one volume of acetone
700272
Nostoc sp. PCC 7120 = FACHB-418
?
-
-
-
?
1.13.11.51
beta-apo-10'-carotenal + O2
assay at pH 7.8, 28°C, incubation stopped by adding one volume of acetone
700272
Nostoc sp. PCC 7120 = FACHB-418
?
-
-
-
?
1.13.11.51
beta-apo-8'-carotenal + O2
assay at pH 7.8, 28°C, incubation stopped by adding one volume of acetone
700272
Nostoc sp. PCC 7120 = FACHB-418
?
-
-
-
?
1.13.11.51
canthaxanthin + O2
assay at pH 7.8, 28°C, incubation stopped by adding one volume of acetone
700272
Nostoc sp. PCC 7120 = FACHB-418
?
-
-
-
?
1.13.11.51
echinenone + O2
assay at pH 7.8, 28°C, incubation stopped by adding one volume of acetone
700272
Nostoc sp. PCC 7120 = FACHB-418
?
-
-
-
?
1.13.11.51
gamma-carotene + O2
assay at pH 7.8, 28°C, incubation stopped by adding one volume of acetone
700272
Nostoc sp. PCC 7120 = FACHB-418
?
-
-
-
?
1.13.11.51
myxol + O2
assay at pH 7.8, 28°C, incubation stopped by adding one volume of acetone
700272
Nostoc sp. PCC 7120 = FACHB-418
?
-
-
-
?
1.13.11.51
myxol 2'-fucoside + O2
assay at pH 7.8, 28°C, incubation stopped by adding one volume of acetone
700272
Nostoc sp. PCC 7120 = FACHB-418
?
-
-
-
?
1.13.11.51
zeaxanthin + O2
assay at pH 7.8, 28°C, incubation stopped by adding one volume of acetone
700272
Nostoc sp. PCC 7120 = FACHB-418
?
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.13.11.51
28
-
at 20°C decreased enzyme acitivity
Nostoc sp. PCC 7120 = FACHB-418
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.13.11.51
7.8
-
assay at
Nostoc sp. PCC 7120 = FACHB-418