Literature summary extracted from

Pajaniappan, M.; Hall, J.E.; Cawthraw, S.A.; Newell, D.G.; Gaynor, E.C.; Fields, J.A.; Rathbun, K.M.; Agee, W.A.; Burns, C.M.; Hall, S.J.; Kelly, D.J.; Thompson, S.A.
A temperature-regulated Campylobacter jejuni gluconate dehydrogenase is involved in respiration-dependent energy conservation and chicken colonization (2008), Mol. Microbiol., 68, 474-491.

Activating Compound

EC Number	Activating Compound	Comment	Organism	Structure
1.1.1.215	additional information	expression and activity of cj0414 and cj0415 are higher at 42°C than at 37°C	Campylobacter jejuni

Application

EC Number	Application	Comment	Organism
1.1.1.215	medicine	ability of Campylobacter jejuni to use gluconate as an electron donor via GADH activity is an important metabolic characteristic that is required for full colonization of avian but not mammalian hosts	Campylobacter jejuni

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
1.1.1.215	mutagenized cj0414 and cj0415 alleles are introduced into Campylobacter jejuni 81-176	Campylobacter jejuni
1.1.99.3	the cj0414-cj0415 PCR product is subcloned into pCRII-TOPO, cj0414 and cj0415 are cloned into the vector pBluescript II	Campylobacter jejuni

Protein Variants

EC Number	Protein Variants	Comment	Organism
1.1.1.215	additional information	cj0414 and cj0415 mutants lack GADH activity at either temperature. Cj0415 mutant does not exhibit gluconate-dependent respiration. Cj0415 mutant is defective in chicken colonization, but exhibits only a minor colonization defect in mice	Campylobacter jejuni
1.1.99.3	81-176cj0414	mutant, lacking the expression of the Cj0414 subunit	Campylobacter jejuni
1.1.99.3	81-176cj0415	mutant, lacking the expression of the Cj0415 subunit	Campylobacter jejuni

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
1.1.1.215	periplasm	-	Campylobacter jejuni	-	-
1.1.99.3	cytoplasm	-	Campylobacter jejuni	5737	-
1.1.99.3	membrane	-	Campylobacter jejuni	16020	-

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
1.1.1.215	26900	-	sequence analysis, cj0414	Campylobacter jejuni
1.1.1.215	63700	-	sequence analysis, cj0415	Campylobacter jejuni
1.1.99.3	26900	-	predicted, subunit Cj0414	Campylobacter jejuni
1.1.99.3	63700	-	predicted, subunit Cj0415	Campylobacter jejuni

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
1.1.99.3	D-gluconate + acceptor	Campylobacter jejuni	-	2-dehydro-D-gluconate + reduced acceptor	-	?
1.1.99.3	D-gluconate + acceptor	Campylobacter jejuni 81-176	-	2-dehydro-D-gluconate + reduced acceptor	-	?
1.1.99.3	D-gluconate + oxidized 2,6-dichlorophenolindophenol	Campylobacter jejuni	activity assay, reaction mixture contains the potential electron acceptors 2,6-dichlorophenolindolphenol, DCIP, and phenazine methosulphate, PMS	2-dehydro-D-gluconate + reduced 2,6-dichlorophenolindophenol	-	?
1.1.99.3	D-gluconate + oxidized 2,6-dichlorophenolindophenol	Campylobacter jejuni 81-176	activity assay, reaction mixture contains the potential electron acceptors 2,6-dichlorophenolindolphenol, DCIP, and phenazine methosulphate, PMS	2-dehydro-D-gluconate + reduced 2,6-dichlorophenolindophenol	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.1.1.215	Campylobacter jejuni	-	-	-
1.1.1.215	Campylobacter jejuni 81-176	-	-	-
1.1.99.3	Campylobacter jejuni	Q0PB96 and Q0PB95	subunit Cj0414 and subunit Cj0415	-
1.1.99.3	Campylobacter jejuni 81-176	Q0PB96 and Q0PB95	subunit Cj0414 and subunit Cj0415	-

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.1.99.3	0.0013	-	cells grown at 37°C, Cj0415 mutant	Campylobacter jejuni
1.1.99.3	0.0019	-	cells grown at 42°C, Cj0414 mutant	Campylobacter jejuni
1.1.99.3	0.0026	-	cells grown at 37°C, Cj0414 mutant	Campylobacter jejuni
1.1.99.3	0.0045	-	cells grown at 42°C, Cj0415 mutant	Campylobacter jejuni
1.1.99.3	0.0141	-	cells grown at 37°C, wild type	Campylobacter jejuni
1.1.99.3	0.0333	-	cells grown at 42°C, wild type	Campylobacter jejuni

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.1.1.215	D-gluconate + NADP+	wild-type exhibits gluconate-dependent respiration. Genes cj0414 and cj0415, orthologous to GADH, are co-transcribed. Campylobacter jejuni 81176 does not use gluconate compounds as sole carbon sources. GADH plays a minor role in mouse colonization	Campylobacter jejuni	2-dehydro-D-gluconate + NADPH + H+	-	?
1.1.1.215	D-gluconate + NADP+	wild-type exhibits gluconate-dependent respiration. Genes cj0414 and cj0415, orthologous to GADH, are co-transcribed. Campylobacter jejuni 81176 does not use gluconate compounds as sole carbon sources. GADH plays a minor role in mouse colonization	Campylobacter jejuni 81-176	2-dehydro-D-gluconate + NADPH + H+	-	?
1.1.99.3	D-gluconate + acceptor	-	Campylobacter jejuni	2-dehydro-D-gluconate + reduced acceptor	-	?
1.1.99.3	D-gluconate + acceptor	-	Campylobacter jejuni 81-176	2-dehydro-D-gluconate + reduced acceptor	-	?
1.1.99.3	D-gluconate + oxidized 2,6-dichlorophenolindophenol	activity assay, reaction mixture contains the potential electron acceptors 2,6-dichlorophenolindolphenol, DCIP, and phenazine methosulphate, PMS	Campylobacter jejuni	2-dehydro-D-gluconate + reduced 2,6-dichlorophenolindophenol	-	?
1.1.99.3	D-gluconate + oxidized 2,6-dichlorophenolindophenol	activity assay, reaction mixture contains the potential electron acceptors 2,6-dichlorophenolindolphenol, DCIP, and phenazine methosulphate, PMS	Campylobacter jejuni 81-176	2-dehydro-D-gluconate + reduced 2,6-dichlorophenolindophenol	-	?

Subunits

EC Number	Subunits	Comment	Organism
1.1.99.3	heterodimer	Cj0414 and Cj0415 subunit	Campylobacter jejuni

Synonyms

EC Number	Synonyms	Comment	Organism
1.1.1.215	GADH	-	Campylobacter jejuni
1.1.1.215	gluconate dehydrogenase	-	Campylobacter jejuni
1.1.99.3	Cj0414/Cj0415	subunit Cj0414 and subunit Cj0415	Campylobacter jejuni
1.1.99.3	GADH	-	Campylobacter jejuni
1.1.99.3	gluconate dehydrogenase	-	Campylobacter jejuni

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
1.1.99.3	25	-	activity assay	Campylobacter jejuni

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
1.1.99.3	6	-	activity assay	Campylobacter jejuni

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.1.1.215	FAD	-	Campylobacter jejuni
1.1.99.3	additional information	a putative NAD, FAD binding site is located within the first 48 amino acids of Cj0415	Campylobacter jejuni

pI Value

EC Number	Organism	Comment	pI Value Maximum	pI Value
1.1.1.215	Campylobacter jejuni	cj0414, isoelectric focusing	-	5.5
1.1.1.215	Campylobacter jejuni	cj0415, isoelectric focusing	-	8.8
1.1.99.3	Campylobacter jejuni	predicted, subunit Cj0414	-	5.5
1.1.99.3	Campylobacter jejuni	predicted, subunit Cj0415	-	8.8

Expression

EC Number	Organism	Comment	Expression
1.1.99.3	Campylobacter jejuni	Cj0414 and Cj0415 are 3.4 and 2.9fold more highly expressed at 42°C compared to 37°C, respectively	up

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Release 2023.1 (January 2023)