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Literature summary extracted from

  • Gescher, J.; Eisenreich, W.; Wörth, J.; Bacher, A.; Fuchs, G.
    Aerobic benzoyl-CoA catabolic pathway in Azoarcus evansii: studies on the non-oxygenolytic ring cleavage enzyme (2005), Mol. Microbiol., 56, 1586-1600.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.2.44 additional information addition of 1 mM thiamine diphosphate to the standard assay causes only a minimal stimulation (8%) Aromatoleum evansii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.44 the boxC gene is expressed in a recombinant Escherichia coli strain as a fusion protein with maltose binding protein (BoxCmal) Aromatoleum evansii

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.2.44 additional information acetoacetyl-CoA (0.2 mM), a potential inhibitor of enoyl-CoA hydratase, has no impact on enzyme activity of BoxCmal. Crotonyl-CoA (0.2 mM), a potential substrate of enoyl-CoA hydratase (crotonase), is neither converted to 3-hydroxybutyryl-CoA nor does it inhibit the standard assay Aromatoleum evansii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.44 0.017
-
 2,3-dihydro-2,3-dihydroxybenzoyl-CoA
-
 Aromatoleum evansii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.2.44 additional information the purified protein does not require divalent metals or any cosubstrates or coenzymes for activity Aromatoleum evansii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.2.44 60000
-
 2 * 60000, SDS-PAGE Aromatoleum evansii
4.1.2.44 120000
-
 gel filtration Aromatoleum evansii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.2.44 2,3-dihydro-2,3-dihydroxybenzoyl-CoA + H2O Aromatoleum evansii the enzyme is involved in the aerobic benzoyl-CoA catabolic pathway. Benzoyl-CoA is oxidized to 2,3-dihydro-2,3-dihydroxybenzoyl-CoA (benzoyl-CoA dihydrodiol) by benzoyl-CoA oxygenase/reductase BoxBA in the presence of molecular oxygen. The next, ring cleaving step is catalysed by BoxC 3,4-dehydroadipyl-CoA semialdehyde + formate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.44 Aromatoleum evansii Q84HH6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.44 wild type and recombinant proteins Aromatoleum evansii

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.2.44 culture condition:benzoate-grown cell
-
 Aromatoleum evansii
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.2.44 4.9
-
-
 Aromatoleum evansii

Storage Stability

EC Number Storage Stability Organism
4.1.2.44 -20°C, the protein can be stored without appreciable loss of activity for months in the presence of 10% (v/v) glycerol Aromatoleum evansii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.44 2,3-dihydro-2,3-dihydroxybenzoyl-CoA + H2O the enzyme is involved in the aerobic benzoyl-CoA catabolic pathway. Benzoyl-CoA is oxidized to 2,3-dihydro-2,3-dihydroxybenzoyl-CoA (benzoyl-CoA dihydrodiol) by benzoyl-CoA oxygenase/reductase BoxBA in the presence of molecular oxygen. The next, ring cleaving step is catalysed by BoxC Aromatoleum evansii 3,4-dehydroadipyl-CoA semialdehyde + formate
-
 ?
4.1.2.44 2,3-dihydro-2,3-dihydroxybenzoyl-CoA + H2O NADPH and semicarbazide are analysed directly by NMR spectroscopy and mass spectrometry. The purified protein does not require molecular oxygen for activity Aromatoleum evansii 3,4-dehydroadipyl-CoA semialdehyde + formate
-
 ?
4.1.2.44 additional information no activity with crotonyl-CoA Aromatoleum evansii ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.2.44 homodimer 2 * 60000, SDS-PAGE Aromatoleum evansii

Synonyms

EC Number Synonyms Comment Organism
4.1.2.44 benzoyl-CoA oxidation component C
-
 Aromatoleum evansii
4.1.2.44 BoxC
-
 Aromatoleum evansii
4.1.2.44 dihydrodiol transforming enzyme
-
 Aromatoleum evansii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.2.44 20
-
 2,3-dihydro-2,3-dihydroxybenzoyl-CoA
-
 Aromatoleum evansii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.44 9
-
-
 Aromatoleum evansii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.2.44 7 11 half maximal activity at pH 7 and pH 11 Aromatoleum evansii

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.2.44 additional information the purified protein does not require divalent metals or any cosubstrates or coenzymes for activity Aromatoleum evansii

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.1.2.44 Aromatoleum evansii
-
-
 5.6