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Literature summary extracted from

  • Blasco, F.; Iobbi, C.; Giordano, G.; Chippaux, M.; Bonnefoy, V.
    Nitrate reductase of Escherichia coli: completion of the nucleotide sequence of the nar operon and reassessment of the role of the alpha and beta subunits in iron binding and electron transfer (1989), Mol. Gen. Genet., 218, 249-256.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.7.5.1
-
 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.5.1 Fe cysteine arrangements typical of iron-sulfur centers are found in the NarH polypeptide. This suggests that the latter is an electron transfer unit of the nitrate reductase complex Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.5.1 25500
-
 x * 138700 + x * 57700, x * 26500, x * 25500, the narGHJI operon that encodes the nitrate reductase encodes four polypeptides NarG (138700 Da), NarH (57700 Da), NarJ (26500 Da) and NarI (25500 Da), calculated from sequence Escherichia coli
1.7.5.1 26500
-
 x * 138700 + x * 57700, x * 26500, x * 25500, the narGHJI operon that encodes the nitrate reductase encodes four polypeptides NarG (138700 Da), NarH (57700 Da), NarJ (26500 Da) and NarI (25500 Da), calculated from sequence Escherichia coli
1.7.5.1 57700
-
 x * 138700 + x * 57700, x * 26500, x * 25500, the narGHJI operon that encodes the nitrate reductase encodes four polypeptides NarG (138700 Da), NarH (57700 Da), NarJ (26500 Da) and NarI (25500 Da), calculated from sequence Escherichia coli
1.7.5.1 138700
-
 x * 138700 + x * 57700, x * 26500, x * 25500, the narGHJI operon that encodes the nitrate reductase encodes four polypeptides NarG (138700 Da), NarH (57700 Da), NarJ (26500 Da) and NarI (25500 Da), calculated from sequence Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.5.1 nitrate + quinol Escherichia coli model of electron transfer in the nitrate reductase: electrons are provided by quinones to the NarI subunit and subsequently transferred to NarH, which eventually delivers them to the molybdenum cofactor where nitrate reduction takes place nitrite + quinone + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.5.1 Escherichia coli P09152 and P11349 and P0AF26 P09152: alpha-chain, P11349: beta-chain, P0AF26: delta-chain
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.5.1 nitrate + quinol
-
 Escherichia coli nitrite + quinone + H2O
-
 ?
1.7.5.1 nitrate + quinol model of electron transfer in the nitrate reductase: electrons are provided by quinones to the NarI subunit and subsequently transferred to NarH, which eventually delivers them to the molybdenum cofactor where nitrate reduction takes place Escherichia coli nitrite + quinone + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.5.1 ? x * 138700 + x * 57700, x * 26500, x * 25500, the narGHJI operon that encodes the nitrate reductase encodes four polypeptides NarG (138700 Da), NarH (57700 Da), NarJ (26500 Da) and NarI (25500 Da), calculated from sequence Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
1.7.5.1 NarGHI
-
 Escherichia coli