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Literature summary extracted from

  • Sanglas, L.; Valnickova, Z.; Arolas, J.L.; Pallares, I.; Guevara, T.; Sola, M.; Kristensen, T.; Enghild, J.J.; Aviles, F.X.; Gomis-Rueth, F.X.
    Structure of activated thrombin-activatable fibrinolysis inhibitor, a molecular link between coagulation and fibrinolysis (2008), Mol. Cell, 31, 598-606.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.17.20
-
 Homo sapiens
3.4.17.20 using the sitting-drop vapor diffusion method. TAFIa conforms to the alpha/beta-hydrolase fold of metallocarboxypeptidases and displays two unique flexible loops on the molecular surface, accounting for structural instability and susceptibility to proteolysis Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.17.20 tick carboxypeptidase inhibitor proteinaceous inhibitor TCI which is found in in the hematophagous ixodid tick Rhipicephalus bursa consists of tandem structurally similar small modules, an N-terminal and a C-terminal domain. TCI inhibits TFAIa mainly through its C-terminal domain. The C-terminal residue His75 is cleaved and the new C-terminus Leu74 approaches and contacts the TAFIa catalytic zinc ion through its carboxylate atoms. In addition to this contact, the C-terminal domain of TCI itneracts with TFAIa through 15 hydrogen bonds and 5 hydrophobic contacts Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.17.20 2.38
-
 hippuryl-Arg Vmax: 44.86 micromol/l/min Homo sapiens
3.4.17.20 3.44
-
 hippuryl-Arg Vmax: 46.05 micromol/l/min Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.17.20 additional information Homo sapiens TFAI binds heparin ?
-
 ?
3.4.17.20 additional information Bos taurus TFAI binds heparin ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.17.20 Bos taurus Q2KIG3
-
-
3.4.17.20 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.17.20
-
 Homo sapiens
3.4.17.20 purified from plasma using a ECH-lysine sepharose column and anion-exchange chromatography Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.17.20 blood
-
 Bos taurus
-
3.4.17.20 blood plasma
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.17.20 hippuryl-Arg + H2O
-
 Homo sapiens hippuric acid + Arg
-
 ?
3.4.17.20 hippuryl-Arg + H2O
-
 Bos taurus hippuric acid + Arg
-
 ?
3.4.17.20 additional information TFAI binds heparin Homo sapiens ?
-
 ?
3.4.17.20 additional information TFAI binds heparin Bos taurus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.17.20 TAFIa
-
 Homo sapiens
3.4.17.20 TAFIa
-
 Bos taurus
3.4.17.20 thrombin-activatable fibrinolysis inhibitor
-
 Homo sapiens
3.4.17.20 thrombin-activatable fibrinolysis inhibitor
-
 Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.17.20 37
-
 assay at Homo sapiens
3.4.17.20 37
-
 assay at Bos taurus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.17.20 2.67
-
 hippuryl-Arg
-
 Homo sapiens
3.4.17.20 2.74
-
 hippuryl-Arg
-
 Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.17.20 7.4
-
 assay at Homo sapiens
3.4.17.20 7.4
-
 assay at Bos taurus