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Literature summary extracted from

  • Thomas, J.L.; Bucholtz, K.M.; Sun, J.; Mack, V.L.; Kacsoh, B.
    Structural basis for the selective inhibition of human 3beta-hydroxysteroid dehydrogenase 1 in human breast tumor MCF-7 cells (2009), Mol. Cell. Endocrinol., 301, 174-182.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.145 17beta-acetoxy-trilostane
-
 Homo sapiens
1.1.1.145 epostane competitive inhibition Homo sapiens
1.1.1.145 trilostane i.e. 2alpha-cyano-4alpha,5alpha-epoxy-17beta-ol-androstane-3-one, competitive inhibition Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.145 0.0037
-
 dehydroepiandrosterone in 0.02 M potassium phosphate buffer, pH 7.4 Homo sapiens
1.1.1.145 0.0341
-
 NAD+ in 0.02 M potassium phosphate buffer, pH 7.4 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.145 42000
-
 SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.145 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.145 MCF-7 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.145 dehydroepiandrosterone + NAD+
-
 Homo sapiens androst-5-en-3,17-dione + NADH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.145 3beta-HSD1
-
 Homo sapiens
1.1.1.145 3beta-hydroxysteroid dehydrogenase 1
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.145 0.055
-
 dehydroepiandrosterone in 0.02 M potassium phosphate buffer, pH 7.4 Homo sapiens
1.1.1.145 0.058
-
 NAD+ in 0.02 M potassium phosphate buffer, pH 7.4 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.145 NAD+
-
 Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.145 0.0001
-
 trilostane in 0.02 M potassium phosphate buffer, pH 7.4 Homo sapiens
1.1.1.145 0.00043
-
 17beta-acetoxy-trilostane in 0.02 M potassium phosphate buffer, pH 7.4 Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.1.1.145 0.0002
-
 in 0.02 M potassium phosphate buffer, pH 7.4 Homo sapiens epostane
1.1.1.145 0.0049
-
 in 0.02 M potassium phosphate buffer, pH 7.4 Homo sapiens trilostane