EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.37 | expressed in Escherichia coli M15 (pREP4) cells | Plasmodium falciparum |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.1.37 | R183a | dimeric mutant with almost wild type activity | Plasmodium falciparum |
1.1.1.37 | R214G | dimeric mutant with almost wild type activity | Plasmodium falciparum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.37 | 0.04954 | - |
oxaloacetate | wild type enzyme | Plasmodium falciparum | |
1.1.1.37 | 0.04955 | - |
3-acetylpyridine-adenine dinucleotide | wild type enzyme | Plasmodium falciparum | |
1.1.1.37 | 0.06186 | - |
oxaloacetate | mutant enzyme R214G | Plasmodium falciparum | |
1.1.1.37 | 0.07238 | - |
NADH | wild type enzyme | Plasmodium falciparum | |
1.1.1.37 | 0.08074 | - |
oxaloacetate | mutant enzyme R183A | Plasmodium falciparum | |
1.1.1.37 | 0.0962 | - |
3-acetylpyridine-adenine dinucleotide | mutant enzyme R214G | Plasmodium falciparum | |
1.1.1.37 | 0.1114 | - |
3-acetylpyridine-adenine dinucleotide | mutant enzyme R183A | Plasmodium falciparum | |
1.1.1.37 | 0.1197 | - |
NADH | mutant enzyme R214G | Plasmodium falciparum | |
1.1.1.37 | 0.1231 | - |
NADH | mutant enzyme R183A | Plasmodium falciparum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.37 | 36100 | - |
4 * 36100, calculated from amino acid sequence | Plasmodium falciparum |
1.1.1.37 | 160000 | - |
enzymatically active preparation of recombinant wild type MDH, calculated from amino acid sequence | Plasmodium falciparum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.37 | (S)-malate + 3-acetylpyridine-adenine dinucleotide | Plasmodium falciparum | can also use 3-acetylpyridine-adenine as cofactor | oxaloacetate + reduced 3-acetylpyridine-adenine dinucleotide + H+ | - |
r | |
1.1.1.37 | (S)-malate + NAD+ | Plasmodium falciparum | highly specific for oxaloacetate and malate | oxaloacetate + NADH + H+ | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.37 | Plasmodium falciparum | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.37 | (S)-malate + 3-acetylpyridine-adenine dinucleotide | can also use 3-acetylpyridine-adenine as cofactor | Plasmodium falciparum | oxaloacetate + reduced 3-acetylpyridine-adenine dinucleotide + H+ | - |
r | |
1.1.1.37 | (S)-malate + NAD+ | highly specific for oxaloacetate and malate | Plasmodium falciparum | oxaloacetate + NADH + H+ | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.37 | homotetramer | 4 * 36100, calculated from amino acid sequence | Plasmodium falciparum |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.37 | L-malate dehydrogenase | - |
Plasmodium falciparum |
1.1.1.37 | MDH | - |
Plasmodium falciparum |
1.1.1.37 | [LDH-like] MDH | - |
Plasmodium falciparum |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.37 | 7280 | - |
3-acetylpyridine-adenine dinucleotide | mutant enzyme R183A | Plasmodium falciparum | |
1.1.1.37 | 7300 | - |
3-acetylpyridine-adenine dinucleotide | wild type enzyme | Plasmodium falciparum | |
1.1.1.37 | 7500 | - |
oxaloacetate | mutant enzyme R214G | Plasmodium falciparum | |
1.1.1.37 | 7880 | - |
NADH | wild type enzyme | Plasmodium falciparum | |
1.1.1.37 | 8320 | - |
oxaloacetate | mutant enzyme R183A | Plasmodium falciparum | |
1.1.1.37 | 8440 | - |
NADH | mutant enzyme R214G | Plasmodium falciparum | |
1.1.1.37 | 8570 | - |
oxaloacetate | wild type enzyme | Plasmodium falciparum | |
1.1.1.37 | 8860 | - |
NADH | mutant enzyme R183A | Plasmodium falciparum | |
1.1.1.37 | 10050 | - |
3-acetylpyridine-adenine dinucleotide | mutant enzyme R214G | Plasmodium falciparum |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.37 | 3-acetylpyridine-adenine dinucleotide | - |
Plasmodium falciparum | |
1.1.1.37 | NADH | - |
Plasmodium falciparum |