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Literature summary extracted from

  • Perez-Pantoja, D.; Donoso, R.N.; Sanchez, M.A.; Gonzalez, B.
    Genuine genetic redundancy in maleylacetate reductase encoding genes involved in degradation of haloaromatic compounds by Cupriavidus necator JMP134 (2009), Microbiology, 155, 3641-3651.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.3.1.32 2,4,6-Trichlorophenol induction of MAR activity in wild type strain Cupriavidus necator
1.3.1.32 2,4-dichlorophenoxyacetate induction of MAR activity in wild type strain Cupriavidus necator

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.32 the hqoD gene is cloned downstream of the arabinose-inducible promoter PBAD in the pBS1 vector to generate pBS1hqoD plasmids and transferred to Pseudomonas putida KT2442 Cupriavidus necator
1.3.1.32 the hxqD gene is cloned downstream of the arabinose-inducible promoter PBAD in the pBS1 vector to generate pBS1hxqD plasmids and transferred to Pseudomonas putida KT2442 Cupriavidus necator
1.3.1.32 the tcpD gene is cloned downstream of the arabinose-inducible promoter PBAD in the pBS1 vector to generate pBS1tcpD, plasmids and transferred to Pseudomonas putida KT2442 Cupriavidus necator

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.32 2,4-dichlorophenoxyacetate + NADH Cupriavidus necator degradation of haloaromatic compounds ?
-
 ?
1.3.1.32 2,4-dichlorophenoxyacetate + NADH Cupriavidus necator JMP 134-1 degradation of haloaromatic compounds ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.32 Cupriavidus necator
-
-
-
1.3.1.32 Cupriavidus necator JMP 134-1
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.32 additional information
-
 basal MAR activity under non-induced conditions in wild type Cupriavidus necator
1.3.1.32 0.332
-
 cell extracts of Pseudomonas putida KT2442 containing the pBS1hxqD plasmid Cupriavidus necator
1.3.1.32 1.112
-
 cell extracts of Pseudomonas putida KT2442 containing the pBS1hqoD plasmid Cupriavidus necator
1.3.1.32 5.983
-
 cell extracts of Pseudomonas putida KT2442 containing the pBS1tcpD plasmid Cupriavidus necator

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.32 2,4-dichlorophenoxyacetate + NADH degradation of haloaromatic compounds Cupriavidus necator ?
-
 ?
1.3.1.32 2,4-dichlorophenoxyacetate + NADH degradation of haloaromatic compounds Cupriavidus necator JMP 134-1 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.1.32 HqoD
-
 Cupriavidus necator
1.3.1.32 HqoD gene product shows MAR activity Cupriavidus necator
1.3.1.32 HxqD
-
 Cupriavidus necator
1.3.1.32 maleylacetate reductase
-
 Cupriavidus necator
1.3.1.32 MAR
-
 Cupriavidus necator
1.3.1.32 TcpD
-
 Cupriavidus necator

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.32 7.5
-
 assay at Cupriavidus necator

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.32 NADH
-
 Cupriavidus necator