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Literature summary extracted from

  • Mora-Montes, H.M.; Bader, O.; Lopez-Romero, E.; Zinker, S.; Ponce-Noyola, P.; Hube, B.; Gow, N.A.; Flores-Carreon, A.
    Kex2 protease converts the endoplasmic reticulum alpha1,2-mannosidase of Candida albicans into a soluble cytosolic form (2008), Microbiology, 154, 3782-3794.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.209 expressed in Escherichia coli Candida albicans

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.209 1-deoxymannojirimycin
-
 Candida albicans
3.2.1.209 swainsonine
-
 Candida albicans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.209 cytosol E-I Candida albicans 5829
-
3.2.1.209 cytosol isoform E-I Candida albicans 5829
-
3.2.1.209 endoplasmic reticulum 65000 Da membrane-bound isoform Candida albicans 5783
-
3.2.1.209 endoplasmic reticulum membrane
-
 Candida albicans 5789
-
3.2.1.209 Golgi vesicle
-
 Candida albicans
-
-
3.2.1.209 Golgi vesicle presence of a 65000 Da membrane-bound isoform that is cleaved by protease Kex2 to soluble 52000 Da isoform E-I Candida albicans
-
-
3.2.1.209 membrane
-
 Candida albicans 16020
-
3.2.1.209 soluble
-
 Candida albicans
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.209 52000
-
 E-I, cytosoloc fraction, Golgi-derived vesicles, SDS-PAGE Candida albicans
3.2.1.209 52000
-
 gel fitlration, isoform E-I Candida albicans
3.2.1.209 65000
-
 membrane bound, SDS-PAGE Candida albicans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.209 Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manalpha(1-2)Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O Candida albicans cytosolic isoform E-I Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + D-mannose
-
 ?
3.2.1.209 Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manalpha(1-2)Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O Candida albicans ATCC 26555 cytosolic isoform E-I Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + D-mannose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.209 Candida albicans Q8J0Q0
-
-
3.2.1.209 Candida albicans Q8J0Q0 strain ATCC26555, CAI4, SC5314, NGY152, HMY5, HMY6
-
3.2.1.209 Candida albicans ATCC 26555 Q8J0Q0 strain ATCC26555, CAI4, SC5314, NGY152, HMY5, HMY6
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.209 proteolytic modification converting via Kex2-protease Candida albicans

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.209
-
 Candida albicans

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.209 0.0908
-
 Candida albicans kex-2-delta-mutant Candida albicans
3.2.1.209 0.0946
-
 Candida albicans strain CAI4 Candida albicans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.209 4-methylumbelliferyl-alpha-D-mannopyranoside + H2O
-
 Candida albicans 4-methylumbelliferone + alpha-D-mannose
-
 ?
3.2.1.209 4-methylumbelliferyl-alpha-D-mannopyranoside + H2O
-
 Candida albicans ATCC 26555 4-methylumbelliferone + alpha-D-mannose
-
 ?
3.2.1.209 Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manalpha(1-2)Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O cytosolic isoform E-I Candida albicans Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + D-mannose
-
 ?
3.2.1.209 Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manalpha(1-2)Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O cytosolic isoform E-I Candida albicans ATCC 26555 Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + D-mannose
-
 ?
3.2.1.209 additional information cytosolic isoform E-I trims free Man8GlcNac2 isomer B into Man7GlcNac2 isomer B Candida albicans ?
-
 ?
3.2.1.209 additional information cytosolic isoform E-I trims free Man8GlcNac2 isomer B into Man7GlcNac2 isomer B Candida albicans ATCC 26555 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.209 monomer 1 * 52000, SDS-PAGE, isoform E-I Candida albicans

Synonyms

EC Number Synonyms Comment Organism
3.2.1.209 alpha1,2-mannosidases
-
 Candida albicans
3.2.1.209 endoplasmic reticulum alpha1,2-mannosidase
-
 Candida albicans

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.2.1.209 0.22
-
-
 Candida albicans 1-deoxymannojirimycin
3.2.1.209 0.54
-
-
 Candida albicans swainsonine