Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Masoumi, A.; Heinemann, I.U.; Rohde, M.; Koch, M.; Jahn, M.; Jahn, D.
    Complex formation between protoporphyrinogen IX oxidase and ferrochelatase during haem biosynthesis in Thermosynechococcus elongatus (2008), Microbiology, 154, 3707-3714.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.3.4 expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH10b Thermosynechococcus vestitus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.3.4 protoporphyrinogen IX + 3 O2 Thermosynechococcus vestitus during heme and chlorophyll biosynthesis, the flavin-dependent protoporphyrinogen IX oxidase catalyzes the six-electron oxidation of protoporphyrinogen IX to form protoporphyrin IX. In the following step, iron is inserted into protoporphyrin IX by ferrochelatase. The two enzymes are organized in an enzyme complex, overview protoporphyrin + 3 H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.3.4 Thermosynechococcus vestitus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.3.3.4 flavoprotein
-
 Thermosynechococcus vestitus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.3.4 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by cobalt affinity chromatography Thermosynechococcus vestitus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.3.4 protoporphyrinogen IX + 3 O2
-
 Thermosynechococcus vestitus protoporphyrin + 3 H2O2
-
 ?
1.3.3.4 protoporphyrinogen IX + 3 O2 during heme and chlorophyll biosynthesis, the flavin-dependent protoporphyrinogen IX oxidase catalyzes the six-electron oxidation of protoporphyrinogen IX to form protoporphyrin IX. In the following step, iron is inserted into protoporphyrin IX by ferrochelatase. The two enzymes are organized in an enzyme complex, overview Thermosynechococcus vestitus protoporphyrin + 3 H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.3.4 More analysis of the enzyme in complex with ferrochelatase by mass spectrometry and immunohistochemical determination, overview Thermosynechococcus vestitus

Synonyms

EC Number Synonyms Comment Organism
1.3.3.4 PPO
-
 Thermosynechococcus vestitus
1.3.3.4 protoporphyrinogen IX oxidase
-
 Thermosynechococcus vestitus

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.3.4 flavin
-
 Thermosynechococcus vestitus

General Information

EC Number General Information Comment Organism
1.3.3.4 metabolism the flavin-dependent protoporphyrinogen IX oxidase catalyzes the six-electron oxidation of protoporphyrinogen IX to form protoporphyrin IX in heme and chlorophyll biosynthesis. In the following step, iron is inserted into protoporphyrin IX by ferrochelatase. The two enzymes are organized in an enzyme complex, overview Thermosynechococcus vestitus